Integrated approach for the characterization,validation and monitoring of protein biomarkers in animal tissue samples as well as on human tissue samples. The ''cell-block'' technique allows the study of cell lines with the same approach. The methodology involves histological and standardized
Last updated on: 12-11-2019 - 11:37
Contact: Cédric Balsat
Organisation: Université Libre de Bruxelles (ULB)
Partners: Université Libre de Bruxelles (ULB)
Status: Published in peer reviewed journal
Epigenetics have taken centre stage in the study of diseases such as cancer, diabetes, and neurodegeneration. A new field is emerging that targets epigenetic modifications for therapeutic intervention: pharmacoepigenetics. Particularly in oncology, inhibitors of epigenetic-modifying proteins, i.e.
Last updated on: 12-11-2019 - 11:34
Contact: Maarten Dhaenens
Organisation: Ghent University (UGent)
Status: Still in development, Internally validated, Published in peer reviewed journal
The aim of these in vitro digestions is to simulate the gastrointestinal digestion of specific food sources and to identify metabolites that might be formed out of this food source by the residing microbiome. For this purpose, fecal samples will be collected from volunteers and will be prepared as
Last updated on: 12-11-2019 - 10:56
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
VITO can offer expertise in animal-free methods for inhalation testing. VITO has an Air-Liquid Interface (ALI) platform with three ALI exposure modules: two commercial systems for bronchial studies and one in-house developed system for lower airway studies. At VITO ALI exposure modules, aerosol
Last updated on: 08-11-2019 - 14:08
Contact: Sandra Verstraelen
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Partners: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: History of use, Internally validated, Published in peer reviewed journal
Cells are treated with compounds and after a specific incubation time the MTT reagent is added. In living cells, the MTT is reduced to the purple formazan. The amount of formazan is related to the amount of living cells. Next, the medium and MTT reagent are aspirated and DMSO is added after which
Last updated on: 08-11-2019 - 14:06
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Transport kinetics accross the skin and/or mucosa is investigated using Franz diffusion cells and LC-UV/MS detection of the investigated molecule in the receptor compartment.
Last updated on: 08-11-2019 - 14:05
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
A compound of interest (e.g. new psychoactive substance, endocrine disrupting compound, ...) is incubated with human liver microsomes and liver cytosolic fractions to generate both Phase I and II metabolites. Samples are prepared for analysis using a simple method in order to avoid possible losses
Last updated on: 08-11-2019 - 14:01
Contact: Philippe Vervliet
Organisation: University of Antwerp (UAntwerpen)
Status: Published in peer reviewed journal
Three-dimensional (3D) lung epithelial cell models mimic key aspects of the parental tissue, including apical-basolateral polarity and barrier function (Barrila et al. 2010, PMID: 20948552). These 3D cultures are generated using the rotating wall vessel (RWV) bioreactor system, allowing host cells
Last updated on: 08-11-2019 - 13:59
Contact: Aurélie Crabbé
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
This method evaluates sprouting propensity of endothelial cells in a collagen matrix. The effect of pro-angiogenic growth factors or co-cultured cells can be measured by quantifying the amount of vascular sprouts that form on endothelial spheroids. Endothelial spheroids are obtained by growing
Last updated on: 08-11-2019 - 13:47
HR MAS analysis of intact tissues consists in the metabolic profiling of entire tumor biopsies using high resolution NMR (nuclear magnetic resonance) using 1H and/or 13C MRS (magnetic resonance spectroscopy). The technique allows combination of metabolomic data with genomic or proteomic data, and
Last updated on: 08-11-2019 - 13:39
Contact: Bénédicte Jordan
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Published in peer reviewed journal
