Ex vivo high-resolution Magnetic Resonance spectroscopy (1H & 13C metabolic profiling) on intact tissues
Commonly used acronym: HR-MAS-MR spectroscopy
Scope of the method
- Animal health
- Human health
- Basic Research
- Translational - Applied Research
- In vitro - Ex vivo
- Human derived cells / tissues / organs
- metabolic profiling
- Magnetic Resonance Spectroscopy
- biopsies analysis
- 1H & 13C-MRS
- cancer research
- tumor metabolism
- cancer diagnosis
- treatment monitoring
- cancer treatment
HR MAS analysis of intact tissues consists in the metabolic profiling of entire tumor biopsies using high resolution NMR (nuclear magnetic resonance) using 1H and/or 13C MRS (magnetic resonance spectroscopy). The technique allows combination of metabolomic data with genomic or proteomic data, and can therefore be used both for exploring the molecular biology of cancer and for clinical improvements in cancer diagnostics, prognostics, treatment planning, and treatment monitoring.
High Resolution NMR (600MHz magnet) equiped with a spinning system for HR-MAS (magic angle spinning) to allow study of intact tissues.
- History of use
- Published in peer reviewed journal
Pros, cons & Future potential
One of the best nondestructive method for study of biopsies composition.
Signal is better when acquired on tissue extracts.
No modifications are planned in the near future.
The technique will remain 'limited' to the analysis of tissue biopsies.
References, associated documents and other information
High-Resolution Magic Angle Spinning (HRMAS) NMR Methods in Metabolomics. Tilgner M, Vater TS, Habbel P, Cheng LL. Methods Mol Biol. 2019;2037:49-67. doi: 10.1007/978-1-4939-9690-2_4.
Contact personBénédicte Jordan
Louvain Drug Research Institute, Nuclear and Electron Spin Technologies platform (NEST)