Computational tissue-based pathology
Scope of the method
- Human health
- Basic Research
- Education and training
- Translational - Applied Research
- In vitro - Ex vivo
- Human derived cells / tissues / organs
- Image analysis
- Machine learning
- Data analysis
- Biomarker validation
- Whole Slide Imaging
- Tissue microarray
- Cell block
- Cell therapy
- Computational pathology
Integrated approach for the characterization,validation and monitoring of protein biomarkers in animal tissue samples as well as on human tissue samples. The ''cell-block'' technique allows the study of cell lines with the same approach. The methodology involves histological and standardized immunohistochemical techniques, whole slide scanning, dedicated image analysis developments, biostatistics and data mining.
- Automated microtome ;
- Automated immunohistochemistry system ;
- Automated tissue micro-arrayer ;
- Whole slide scanner ;
- Image analysis software packages.
- Published in peer reviewed journal
Pros, cons & Future potential
Standardized laboratory procedures and quality controls ensure reproducibility and traceability. Brightfield IHC has the advantage to preserve tissue morphology and thus antigen location at histological and cell levels. By simultaneously processing thousands of samples, the TMA technology allows standardized screening of protein expression using IHC and thus provides a very efficient way for biomarker validation. Slide scanning and image analysis enable archiving, sharing, quantitative staining characterization and colocalization analysis. Finally, data analysis enables biomarkers to be statistically validated and compared.
- Time consuming ;
- Multidisciplinar expertise ;
- Standardization requirement.
In constant development of methods dedicated to new issues.
- Immunology ;
- Drug development (companion tests) ;
- Animal health.
References, associated documents and other information
Cfr. associated document.
Tissue-based biomarker colocalization
Method development is the result of a longstanding collaboration between the Pathology Department of the Erasme Hospital (Brussels) and the LISA (Laboratory of Image Synthesis and Analysis) of the Brussels School of Engineering (ULB).
Contact personCédric Balsat
OrganisationsUniversité libre de Bruxelles
CMMI - DIAPath