Transdermal and transmucosal kinetics using FDC

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Scope of the method

The Method relates to
  • Human health
The Method is situated in
  • Basic Research
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
Skin

Description

Method keywords
  • skin absorption
  • transport
  • kinetics
  • LC-MS
Scientific area keywords
  • pharmacokinetics
  • toxicokinetics
  • toxicity
Method description

Transport kinetics accross the skin and/or mucosa is investigated using Franz diffusion cells and LC-UV/MS detection of the investigated molecule in the receptor compartment.

Lab equipment
  • Franz diffusion cell system ;
  • LC-UV/MS.
Method status
  • Internally validated
  • Published in peer reviewed journal

Pros, cons & Future potential

Advantages

No in vitro cell line is used but real ex vivo skin or mucosa.

References, associated documents and other information

References

Taevernier L, et al. (2016). Human skin permeation of emerging mycotoxins (beauvericin and enniatins). J Expo Sci Environ Epidemiol. 26(3):277-87.

Taevernier L, et al. (2015). Enniatin-containing solutions for oromucosal use: Quality-by-design ex-vivo transmucosal risk assessment of composition variability. Int J Pharm. 491(1-2):144-51.

Veryser L, et al. (2014). Quantitative transdermal behavior of pellitorine from Anacyclus pyrethrum extract. Phytomedicine. 21(14):1801-7.

Veryser L, et al. (2016). Mucosal and blood-brain barrier transport kinetics of the plant N-alkylamide spilanthol using in vitro and in vivo models. BMC Complement Altern Med. 16:177.

Associated documents

Contact person

Yorick Janssens

Organisations

Ghent University (UGent)
Pharmaceutical analysis
Belgium