Pharmaceutical products intended for parenteral use must be free from pyrogenic (fever–inducing) contamination. Pyrogens comprise endotoxin from Gram-negative bacteria and non-endotoxin pyrogens (NEP) from Gram-positive bacteria, viruses, and fungi. The longstanding compendial test for pyrogens is

Last updated on: 29-01-2025 - 10:44

Organisation: Janssen Pharma of JNJ
Partners: Janssen Pharma of JNJ
Status: Internally validated, Published in peer reviewed journal
The BALB/c-derived - 4T1 mammary tumor cell line and RAW264.7 macrophage cell line were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin in culture flasks. Harvesting of cultured

Last updated on: 28-01-2025 - 16:41

Contact: Kristel Demeyere
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Identifying drug-target interactions is a crucial step in drug repositioning, the process of suggesting new indications for known drugs. There are about 9000 FDA-approved and experimental small molecule drugs and more than 500.000 protein records available. Performing in vitro experiments would be

Last updated on: 28-01-2025 - 16:41

Contact: Daniele Parisi
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Biotech/TU-Dresden, Max-Planck-Institut für Informatik Saarbrucken
Status: Still in development
Human embryonic kidney (HEK) 293 FT cells is a celline that is very easy to culture and is used to obtain high viral titers. “293” is a reference to the 293th experiment wherein the cell line was discovered. A transfection with an adenovirus type 5 DNA fragment took place, causing the cell line to

Last updated on: 28-01-2025 - 15:12

Contact: Matthias Rombaut
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
The Chorioallantoic Membrane (CAM) assay is a versatile, cost-effective in ovo model using the vascular-rich membrane of fertilized chicken eggs to study biological processes such as angiogenesis, tumor growth, metastasis, and drug testing. Its transparency and rapid vascularization make it ideal

Last updated on: 19-12-2024 - 11:45

Contact: Lotte Alders
Organisation: University of Hasselt (UHasselt)
Status: History of use, Published in peer reviewed journal
Human duodenal biopsies from metabolic dysfunction-associated steatohepatitis (MASH) patients were used to generate organoids and then investigate potential alterations in intestinal barrier and absorptive functions.

Last updated on: 19-12-2024 - 09:15

Organisation: Université Libre de Bruxelles (ULB)
Partners: Université Libre de Bruxelles (ULB)
Status: Published in peer reviewed journal
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were seeded in 48-well multielectrode array (MEA) plates and were treated with four doses of reference compounds (covering and exceeding clinical free plasma peak concentrations) and MEA recordings were conducted for 4 days.

Last updated on: 13-12-2024 - 14:46

Contact: Cannot be disclosed
Organisation: Janssen Pharma of JNJ
Status: Internally validated, Published in peer reviewed journal
Design and fabrication of a method to enhance the cost-effectiveness of organoid culturing and drug screening assays by miniaturizing the cultures and reduce the required reagent volumes to the sub-nanoliter range through microfluidic techniques. By growing single or multiple organoids per microbead

Last updated on: 02-12-2024 - 15:51

Contact: Cannot be disclosed
Organisation: Cannot be disclosed
Status: Still in development
Throughout a woman's life, the vaginal epithelium is subject to strong physiological variations which, combined with the use of external chemical molecules, can lead to intimate discomforts such as irritation, dryness and dysbiosis. The safety of these external agents, whether hygiene or skincare

Last updated on: 02-12-2024 - 09:28

Contact: Christel Boudry
Organisation: straticell
Status: Still in development
The protocol is aimed at developing primary human breast organoids that have a morphology similar to the one observed in the in vivo breast. This morphology encompasses a complex network organization composed of interconnected branches that terminate in TDLU-like structures. The organoids are

Last updated on: 11-10-2024 - 15:19

Contact: Colinda Scheele
Organisation: Katholieke Universiteit Leuven (KUL), Oncology - KU Leuven
Status: Still in development, History of use, Published in peer reviewed journal