We established a protocol to efficiently generate hepatic stellate cells (HSCs) from human pluripotent stem cells (PSCs). Our procedure generated complex in vitro spheroid cultures that better mimic the complexity of the liver as well as liver function. In co-culture, iPSC-HSCs promote maintenance

Last updated on: 04-11-2020 - 12:32

Organisation: Vrije Universiteit Brussel
Partners: KU Leuven
Status: Published in peer reviewed journal
In this method, we use rabbit monoclonal antibodies that are produced in vitro to identify xenografted human stem cell-derived hepatocytes in frozen murine liver slices. These recombinant antibodies were produced in vitro by cloning antibody genes for immune-specific heavy and light antibody chains

Last updated on: 16-10-2020 - 11:27

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use
Intestinal organoids are cultured from intestinal biopsies obtained during routine endoscopy. The stem cell containing crypts are isolated and cultured in a 3D ECM (Matrigel) in the presence of the desired growth factors. The present stem cells will expand and give rise to all epithelial cells of

Last updated on: 15-10-2020 - 14:32

Contact: Cannot be disclosed
Organisation: KU Leuven
Status: Published in peer reviewed journal
Alkaline comet assay (COM) and Micronucleus test (MN) are well-established cytogenetic tests that are used to detect both immediate damages i.e. DNA fragmentation, and permanent damage which results in micronuclei formation. These techniques are often applied separately or, sometimes, in combination

Last updated on: 08-10-2020 - 10:59

Contact: Ha Nguyen
Organisation: Sciensano, Université libre de Bruxelles
Partners: Liège Université
Status: History of use, Internally validated
The CALUX method is an in vitro bioassay that uses reporter gene cell lines that have been stably transfected with a luciferase reporter gene under the control of relevant receptor specific DNA response element. This enables the screening for chemicals that can bind to specific receptors and

Last updated on: 17-09-2020 - 11:49

Contact: Imke Boonen
Organisation: Vrije Universiteit Brussel
Metabolomics and 13C tracer analysis are state-of-the-art techniques that allow determining the concentration of metabolites and the activity of metabolic pathways, respectively. Three dimensional (3D) cultures of cancer cells constitute an enriched in vitro environment that can be used to assay

Last updated on: 27-08-2020 - 14:42

Contact: Dorien Broekaert
Organisation: VIB KU Leuven, KU Leuven
Status: Published in peer reviewed journal
Isolation Femoral and tibial bones are isolated from 10-12 weeks old C57BL/6J mice. After cutting the bone's ends, the bone marrow is flushed out using a 23G syringe with PBS into a 50ml falcon tube. After centrifugation cells are re-suspended in FCS (0.5ml/mouse leg). Differentiation 0.5ml of the

Last updated on: 14-08-2020 - 15:26

Contact: Jerome Hendriks
Organisation: Hasselt University
Status: History of use, Internally validated, Published in peer reviewed journal
Characterization of Human Oligodendroglia derived-EV-rich in sHSPs (HOG-EV-sHSPs). Establish HSPB1/B8 stably expressing cell lines for the production of sHSPs rich-EV cell culture conditioned medium. Human Oligodendroglia cell lines (HOG) were stably transduced with pLenti-HSPBs gene constructs

Last updated on: 14-08-2020 - 15:22

Contact: Joy Irobi
Organisation: Hasselt University
Status: Still in development, Internally validated, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
The feed undergoes an in vitro enzymatic hydrolysis simulating the digestion in the upper digestive tract followed by a dialysis mimicking the intestinal absorption. Finally, an in vitro batch fermentation is performed and simulates the fermentation occurring in the hindgut. The ingredients, ground

Last updated on: 16-07-2020 - 15:04

Contact: Nadia Everaert
Organisation: Liège Université
Status: Published in peer reviewed journal