We developed a high-throughput live-cell imaging-based organoid analysis platform, Organoid Brightfield Identification-based Therapy Screening (Orbits), that allows for kinetic monitoring of organoid growth and drug responses in routine extracellular matrix domes, high-throughput 384-well
Last updated on: 16-03-2022 - 13:59
Contact: Christophe Deben
Organisation: University of Antwerp (UAntwerpen)
Status: Internally validated
Quantitative Structure Activity Relationship modeling is generally used to construct models in which molecular descriptors of chemical compounds are used to predict endpoints/activities of interest. Commercial packages are available that can be implemented, but new models can be constructed if
Last updated on: 16-03-2022 - 13:49
Contact: Geert Verheyen
Organisation: Thomas More University of Applied Sciences
Status: Still in development, History of use, Published in peer reviewed journal
The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration
Last updated on: 28-02-2022 - 11:04
Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
Albumin secretion in the supernatant of in vitro cultures can be measured by an enzyme-linked immunosorbent assay. The assay is based upon a quantitative sandwich enzyme immunoassay technique and measures albumin in the culture medium of primary hepatocytes and/or hepatocyte like cells. Standards
Last updated on: 28-02-2022 - 11:02
This method provides a new and easy to standardize enzymatic isolation protocol to obtain human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs). hUC-MSCs are obtained within 3 hours and the isolation method provides a minimal risk of bacterial contamination. The so-obtained hUC-MSCs were
Last updated on: 28-02-2022 - 11:02
Contact: Karolien Buyl
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
Human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs) express several key liver-specific transcription factors as well as hepatic progenitor markers. However, they still lack the hepatocyte nuclear factors 1-alpha (HNF1a) and 4-alpha (HNF4a), indispensable for their reprogramming towards
Last updated on: 28-02-2022 - 11:02
HepG2 is a human hepatoma derived cell line, which are epithelial in morphology. It was established from liver tissue of a 15-year-old Caucasian male with a well differentiated hepatocellular carcinoma. The HepG2 cell line is one of the most used human liver-based in vitro models. The cells secrete
Last updated on: 28-02-2022 - 11:02
Cryopreserved differentiated HepaRG cells (obtained from Biopredic International) are derived from a human hepatocellular carcinoma. These cells are an unique in vitro tool that provides reproducible results and exhibit many characteristics of primary human hepatocytes such as morphology, expression
Last updated on: 28-02-2022 - 11:02
In this method, we use rabbit monoclonal antibodies that are produced in vitro to identify xenografted human stem cell-derived hepatocytes in frozen murine liver slices. These recombinant antibodies were produced in vitro by cloning antibody genes for immune-specific heavy and light antibody chains
Last updated on: 28-02-2022 - 11:02
Rat liver epithelial cells are cultivated at 100% confluency on 100 µg/mL rat tail collagen type I coated culture dishes in base medium and sequentially exposed to hepatogenic growth factors and cytokines. Base medium consisted of William’s E medium without glutamine supplemented with 7.33 IE/mL
Last updated on: 28-02-2022 - 11:02
Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
