Measurement of Albumin Secretion as Functionality Test

Scope of the method

The Method relates to
  • Human health
The Method is situated in
  • Basic Research
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
parenchymal liver cells, stem cell-derived hepatocyte-like cells


Method keywords
  • albumin
Scientific area keywords
  • in vitro cell culture
  • hepatic in vitro model
  • hepatic differentiation
  • liver cells
Method description

Albumin secretion in the supernatant of in vitro cultures can be measured by an enzyme-linked immunosorbent assay. The assay is based upon a quantitative sandwich enzyme immunoassay technique and measures albumin in the culture medium of primary hepatocytes and/or hepatocyte like cells. Standards and samples are sandwiched between an immobilized polyclonal antibody and a biotinylated polyclonal antibody specific for albumin. The latter is recognized by a streptavidinperoxidase conjugate and after adding a peroxidase enzyme substrate, a blue color is developed. The blue product, on its turn, is converted towards a yellow derivative of which the absorbance can be measured at 450 nm.

Lab equipment

Multiplate reader.

Method status
  • History of use

Pros, cons & Future potential


Easy to use.

References, associated documents and other information


Bolleyn et al. Effect of Trichostatin A on miRNA expression in cultures of primary rat hepatocytes. Toxicol In Vitro. 2011 Sep;25(6):1173-82

Contact person

Steven Branson


Vrije Universiteit Brussel (VUB)
Pharmaceutical and Pharmacological Sciences
In Vitro Toxicology and Dermato-Cosmetology
Brussels Region