TEM, NTA and western blot were used to characterize the purified inflammation‐associated EV subpopulations from TNF-α treated HUVEC based on their sedimentation speeds (10K and110K) and surface markers (CDs and ICAM-1). Protein arrays were used to discover the immunomodulatory content of
Last updated on: 14-11-2019 - 14:02
Contact: Baharak Hosseinkhani
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
FIBEr (Flanders Institute for Biomechanical Experimentation) is a laboratory that investigates the mechanical properties (stiffness, strength, microstructure) of biological tissue, in casu bones, muscles, ligaments, blood vessels, etc. A quantitative characterization of the mechanical behavior of
Last updated on: 12-11-2019 - 11:45
Contact: Nele Famaey
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Still in development, History of use, Internally validated
Integrated approach for the characterization,validation and monitoring of protein biomarkers in animal tissue samples as well as on human tissue samples. The ''cell-block'' technique allows the study of cell lines with the same approach. The methodology involves histological and standardized
Last updated on: 12-11-2019 - 11:37
Contact: Cédric Balsat
Organisation: Université Libre de Bruxelles (ULB)
Partners: Université Libre de Bruxelles (ULB)
Status: Published in peer reviewed journal
Epigenetics have taken centre stage in the study of diseases such as cancer, diabetes, and neurodegeneration. A new field is emerging that targets epigenetic modifications for therapeutic intervention: pharmacoepigenetics. Particularly in oncology, inhibitors of epigenetic-modifying proteins, i.e.
Last updated on: 12-11-2019 - 11:34
Contact: Maarten Dhaenens
Organisation: Ghent University (UGent)
Status: Still in development, Internally validated, Published in peer reviewed journal
The aim of these in vitro digestions is to simulate the gastrointestinal digestion of specific food sources and to identify metabolites that might be formed out of this food source by the residing microbiome. For this purpose, fecal samples will be collected from volunteers and will be prepared as
Last updated on: 12-11-2019 - 10:56
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
VITO can offer expertise in animal-free methods for inhalation testing. VITO has an Air-Liquid Interface (ALI) platform with three ALI exposure modules: two commercial systems for bronchial studies and one in-house developed system for lower airway studies. At VITO ALI exposure modules, aerosol
Last updated on: 08-11-2019 - 14:08
Contact: Sandra Verstraelen
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Partners: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: History of use, Internally validated, Published in peer reviewed journal
Three-dimensional (3D) lung epithelial cell models mimic key aspects of the parental tissue, including apical-basolateral polarity and barrier function (Barrila et al. 2010, PMID: 20948552). These 3D cultures are generated using the rotating wall vessel (RWV) bioreactor system, allowing host cells
Last updated on: 08-11-2019 - 13:59
Contact: Aurélie Crabbé
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
HR MAS analysis of intact tissues consists in the metabolic profiling of entire tumor biopsies using high resolution NMR (nuclear magnetic resonance) using 1H and/or 13C MRS (magnetic resonance spectroscopy). The technique allows combination of metabolomic data with genomic or proteomic data, and
Last updated on: 08-11-2019 - 13:39
Contact: Bénédicte Jordan
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Published in peer reviewed journal
Sandwich-cultured hepatocytes are exposed to test compounds (e.g. drug candidates) or known hepatotoxicants (as controls) both in the absence and in the presence of a mixture of physiologically relevant bile acids. After 24h, decreased hepatocyte viability and functionality in the presence of bile
Last updated on: 08-11-2019 - 13:35
Contact: Pieter Annaert
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Internally validated, Published in peer reviewed journal
This method is used to monitor cardiac cell cultures in vitro. It can be used for monitoring both cell activity and drug-induced cardiac side-effects for primary cardiomyocytes or hIPSC-derived cardiomyocytes. Unlike conventional microelectrode array systems that only measure the local field
Last updated on: 08-11-2019 - 13:34
Contact: Thomas Pauwelyn
Organisation: Interuniversitair Micro-Electronica Centrum (IMEC)
Status: Published in peer reviewed journal
