Heart-on-chip monitoring of cardiomyocyte electrical activity
Commonly used acronym: Microelectrode arrays
Scope of the method
- Animal health
- Human health
- Basic Research
- Translational - Applied Research
- In vitro - Ex vivo
- Human derived cells / tissues / organs
- action potentials
- local field potentials
- induced pluripotent stem cells
- cardiac toxicity
- drug testing
- in vitro
- cardiac toxicity
- drug screening
- in vitro cell culture
This method is used to monitor cardiac cell cultures in vitro. It can be used for monitoring both cell activity and drug-induced cardiac side-effects for primary cardiomyocytes or hIPSC-derived cardiomyocytes. Unlike conventional microelectrode array systems that only measure the local field potentials around cardiac cells, the imec-developed microelectrode array monitors the extracellular electrical activity, intracellular (open-cell) action potentials, and impedance of cells cultured directly on the chip. Furthermore, this method offers high throughput recordings of cells: the CMOS-based chip contains 16384 recording electrodes, wherein 1024 channels can simultaneously record multimodal cell electrophysiology on single-cell level. This multimodal MEA may open up new opportunities in high-throughput cell-based pharmacological screening as well as fundamental studies of cells.
Imec-developed microelectrode array chips and read-out setup.
- Published in peer reviewed journal
Pros, cons & Future potential
High quality electrical recordings (intracellular action potential recordings) High spatio-temporal resolution Single-cell recordings 16,384 recording electrodes Pacing Multimodal Structured chip surfaces.
- Custom-made tools ;
- Large volume of data ;
- Complex chip fabrication.
- Software upgrades for user-friendliness ongoing ;
- Hardware improvements.
- Brain-on-chip ;
- Cancer-on-chip ;
- Gut-on-chip ...
References, associated documents and other information
Single-cell recording and stimulation with a 16k micro-nail electrode array int…
Open-cell recording of action potentials using active electrode arrays
Action potential-based MEA platform for in vitro screening of drug-induced card…
Put your heart on this chip and your cancer treatment becomes ‘healthier’
Contact personThomas Pauwelyn
Life Science Technologies