Inflammatory extracellular vesicles

Scope of the method

Alternative method relates to
  • Human health
Alternative method is situated in
  • Basic Research
  • Translational - Applied Research
Type of alternative method
  • In vitro - Ex vivo
This method makes use of
  • Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
HUVEC/ THP-1

Description

Method keywords
  • EV isolation
  • EV charactrization
  • profiling
Scientific area keywords
  • cardiovascular disorders
Method description

TEM, NTA and western blot were used to characterize the purified inflammation‐associated EV subpopulations from TNF-α treated HUVEC based on their sedimentation speeds (10K and110K) and surface markers (CDs and ICAM-1). Protein arrays were used to discover the immunomodulatory content of subpopulations. In addition, functional integrity of the EV subpopulations was assessed using migration cell based assays.

Lab equipment
  • NTA 
  • TEM 
  • WB
  • Protein array
  • SPR
  • FACs
Method status
  • Published in peer reviewed journal
  • Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)

References, associated documents and other information

Associated documents

Contact person

Baharak Hosseinkhani

Organisations

Hasselt University
Bionanotechnology Research Group, Biomedical Research Institute (BIOMED), Hasselt University, Martelarenlaan 42, B-3500 Hasselt, Belgium
Belgium
Flemish Region