We describe a fluorescent cell-based in vitro infection model that reproduces host-Batrachochytrium dendrobatidis (Bd) interactions. Using the epithelial cell line A6 from Xenopus laevis, we reproduced different stages of host cell infection and intracellular growth of Bd, resulting in host cell
Last updated on: 10-01-2020 - 15:28
Contact: Elin Verbrugghe
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
We have successfully set-up the iPSC technology and are able to derive human cortical neurons for the study of neurodevelopmental disorders e.g. the MECP2 duplication syndrome (published) and other projects in the lab (ongoing). We also create isogenic lines using CrispR-Cas technology. All lines
Last updated on: 07-01-2020 - 15:23
Contact: Hilde Van Esch
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, History of use, Published in peer reviewed journal
In view of safety of pregnant women, a promising in vitro zebrafish embryo developmental toxicity assay has been developed to test pharmaceutical and chemical compounds for their teratogenic potential. The protocol deals with exposing zebrafish embryos to a range of compound concentrations at 28°C
Last updated on: 06-01-2020 - 16:55
Contact: Steven Van Cruchten
Organisation: University of Antwerp (UAntwerpen)
Status: Still in development, Published in peer reviewed journal
The ex-vivo endothelium-stent contact model allows measuring the impact of implants on the arterial tissue. The endothelium is directly exposed by inserting a wire made of the investigated material into the lumen of the aortic ring to mimic the contact between the coronary stent and the endothelium.
Last updated on: 03-12-2019 - 17:23
Contact: Eleonora Scarcello
Organisation: Université Catholique de Louvain (UCL)
Partners: Université Catholique de Louvain (UCL)
Status: Internally validated
The lab routinely use C. elegans culture as genetic model. We explore the development and the ageing of the neuromuscular system. The methods used regularly in our lab include molecular biology, generation of transgenic animals or mutants, crossings, genetic screen, calcium imaging, fluorescence
Last updated on: 02-12-2019 - 13:54
Contact: patrick laurent
Organisation: Université Libre de Bruxelles (ULB)
Status: Internally validated, Published in peer reviewed journal
The cytokinesis-block micronucleus method allows assessing the presence of DNA damage at the chromosome level. It is an essential part of toxicology, because mutation is a crucial event in carcinogenesis. The capacity of inhaled particles to induce irreversible mutations in type II lung epithelial
Last updated on: 26-11-2019 - 17:35
Contact: Violaine Sironval
Organisation: Université Catholique de Louvain (UCL)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
Cell Culture and Reagents hiPSC-CMs can be obtained commercially either as living pre-plated cells seeded onto fibronectin-coated 96-well mClear plates (Greiner Bio-One, No. 655090) or can be plated in house at a density (~25,000 cells/well) suited to forming a confluent synchronously beating mono
Last updated on: 26-11-2019 - 13:17
Contact: Ard Teisman
Organisation: Janssen Pharma of JNJ
Status: Internally validated, Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The BALB/c-derived - 4T1 mammary tumor cell line and RAW264.7 macrophage cell line were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin in culture flasks. Harvesting of cultured
Last updated on: 26-11-2019 - 13:12
Contact: Kristel Demeyere
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Worldwide, outdoor air pollution is responsible for 4.2 million premature deaths per year. Both chronic and acute exposure to particulate matter air pollution is a risk factor for heart and lung diseases. One of the atmospheric pollutant particles is represented by soot or black carbon (BC)
Last updated on: 15-11-2019 - 16:16
Contact: Eva Bongaerts
Organisation: University of Hasselt (UHasselt)
Partners: University of Hasselt (UHasselt), Katholieke Universiteit Leuven (KUL)
Status: History of use, Internally validated, Published in peer reviewed journal
The hemolysis assay remarkably predicts the inflammatory potential of inhaled particles. The capacity of particles to damage cellular membranes is a key property to predict their inflammatory potential upon inhalation. In macrophages and epithelial cells exposed to particles, alteration of the
Last updated on: 15-11-2019 - 16:15
Contact: Dominique Lison
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
