Cytokinesis-block micronucleus method
Commonly used acronym: CBMN
Scope of the method
- Human health
- Basic Research
- In vitro - Ex vivo
- Animal derived cells / tissues / organs
- DNA damage
- chromosomal lesions
- lung epithelial cells
- fluorescence microscope
- inhaled particles
The cytokinesis-block micronucleus method allows assessing the presence of DNA damage at the chromosome level. It is an essential part of toxicology, because mutation is a crucial event in carcinogenesis. The capacity of inhaled particles to induce irreversible mutations in type II lung epithelial cells is evaluated in this assay. By exposing type II lung epithelial cells to particles and blocking the cytokinesis, micronuclei can be scored in divided cells and are the reflection of mutations induced by particles.
- Validated by an external party (e.g. OECD, EURL ECVAM,…)
Pros, cons & Future potential
- Great predictivity ;
- Sensitivity ;
- Precision ;
Other cell lines can be used
References, associated documents and other information
Fenech, M., Cytokinesis-block micronucleus cytome assay. Nat Protoc, 2007. 2(5): p. 1084-104.
Fenech, M., The in vitro micronucleus technique. Mutat. Res, 2000. 455(1-2): p. 81-95.
Contact personViolaine Sironval
The Louvain Centre for Toxicology and Applied Pharmacology