It is the goal of the DNA-adductomics to search for DNA-adducts that might be formed during interaction with contaminants. The analysis of DNA adducts is performed using ultra-high performance liquid chromatography coupled to hybrid quadrupole-Orbitrap high resolution mass spectrometry. Both the
Last updated on: 12-11-2019 - 11:16
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
Our polar metabolomics profiling and fingerprinting methodology applies ultra-high performance liquid chromatography coupled to hybrid quadrupole-Orbitrap high resolution mass spectrometry. Both the instrumental method, as well as generic extraction protocols for colon tissue, cell cultures, urine,
Last updated on: 12-11-2019 - 11:13
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
The aim of these in vitro digestions is to simulate the gastrointestinal digestion of specific food sources and to identify metabolites that might be formed out of this food source by the residing microbiome. For this purpose, fecal samples will be collected from volunteers and will be prepared as
Last updated on: 12-11-2019 - 10:56
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Our lipidomics profiling and fingerprinting methodology enables the analysis of all lipids within a biological matrix. These lipids can then be compared between an individual or condition with a specific metabolic state and healthy controls to find biomarkers or pathways that can be related to
Last updated on: 08-11-2019 - 14:12
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: History of use, Internally validated, Published in peer reviewed journal
Cells are treated with compounds and after a specific incubation time the MTT reagent is added. In living cells, the MTT is reduced to the purple formazan. The amount of formazan is related to the amount of living cells. Next, the medium and MTT reagent are aspirated and DMSO is added after which
Last updated on: 08-11-2019 - 14:06
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Molecules are descripted by hundreds to thousands different descriptors (e.g. molecular weight, pI, log P,...) after which they can be clustered in different classes.
Last updated on: 08-11-2019 - 14:05
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Transport kinetics accross the skin and/or mucosa is investigated using Franz diffusion cells and LC-UV/MS detection of the investigated molecule in the receptor compartment.
Last updated on: 08-11-2019 - 14:05
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
Three-dimensional (3D) lung epithelial cell models mimic key aspects of the parental tissue, including apical-basolateral polarity and barrier function (Barrila et al. 2010, PMID: 20948552). These 3D cultures are generated using the rotating wall vessel (RWV) bioreactor system, allowing host cells
Last updated on: 08-11-2019 - 13:59
Contact: Aurélie Crabbé
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The Galleria mellonella assay makes use of larvae of the Greater Wax Moth. G. mellonella larvae are injected with a small amount of microorganisms, and survival can easily be followed for up to 1 week. This allows to compare virulence between different organisms. Alternatively, after injection of
Last updated on: 08-11-2019 - 13:58
Contact: Tom Coenye
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The C. elegans assay can be used to study the virulence of various microorganisms, and can be used to evaluate the antimicrobial effect of drugs or drug candidates. The two main outcome parameters are (i) survival of C. elegans and (ii) microbial load.
Last updated on: 08-11-2019 - 13:57
Contact: Tom Coenye
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
