Galleria mellonella screening assay in microbiology

Scope of the method

Alternative method relates to
  • Human health
Alternative method is situated in
  • Basic Research
  • Education and training
  • Translational - Applied Research
Type of alternative method
  • In vivo
This method makes use of
  • Animal derived cells / tissues / organs
Used species
Galleria mellonella

Description

Method keywords
  • microbiology
  • infection
  • virulence
  • antibiotics
  • biofilm
Scientific area keywords
  • microbiology
  • infection
  • virulence
  • antibiotics
  • biofilm
Method description

The Galleria mellonella assay makes use of larvae of the Greater Wax Moth. G. mellonella larvae are injected with a small amount of microorganisms, and survival can easily be followed for up to 1 week. This allows to compare virulence between different organisms. Alternatively, after injection of the microorganisms a treatment can be administered, to determine antimicrobial activity.

Lab equipment

Basic microbiology lab equipment.

Method status
  • Published in peer reviewed journal

References, associated documents and other information

Associated documents

Contact person

Tom Coenye

Organisations

Ghent University
Laboratory of Pharmaceutical Microbiology
Belgium