The method enables to quantitatively assess the invasion of endothelial cells in extracellular-matrix mimicking hydrogels, such as collagen or polyethylene glycol, and to measure the forces exerted by the cells that enable them to invade. Endothelial cells are seeded on the side of a hydrogel and
Last updated on: 11-02-2020 - 08:55
Contact: Hans Van Oosterwyck
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Patient-derived explants of prostate cancer provide an ex vivo model that retains the architecture and microenvironment of the native tissue. It enables the evaluation of drug responses on individual patient’s tumors ex vivo without passaging in animals. It is compatible with all molecular analysis
Last updated on: 07-02-2020 - 15:55
Contact: Johan Swinnen
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: University of Adelaide
Status: History of use, Published in peer reviewed journal
Ex vivo lung perfusion (EVLP) is a form of isolated lung perfusion in normothermic conditions and can be achieved with a pump-driven perfusion machine that recirculates a preservation solution through the vasculature of the lung in addition to mechanical ventilation. EVLP is based on a physiological
Last updated on: 07-02-2020 - 15:50
Contact: Arne Neyrinck
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We have developed an innovative two-chamber skin explant model specifically for studies involving skin of marine fish. An apparatus was 3D-printed and consists of two plates, a lower and upper plate (with funnel). By mounting the explant between the plates, two chambers can be created with the
Last updated on: 04-02-2020 - 13:31
Contact: Maaike Vercauteren
Organisation: Ghent University (UGent), University of Liège (ULiège)
Status: Internally validated
We describe a fluorescent cell-based in vitro infection model that reproduces host-Batrachochytrium dendrobatidis (Bd) interactions. Using the epithelial cell line A6 from Xenopus laevis, we reproduced different stages of host cell infection and intracellular growth of Bd, resulting in host cell
Last updated on: 10-01-2020 - 15:28
Contact: Elin Verbrugghe
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
We have successfully set-up the iPSC technology and are able to derive human cortical neurons for the study of neurodevelopmental disorders e.g. the MECP2 duplication syndrome (published) and other projects in the lab (ongoing). We also create isogenic lines using CrispR-Cas technology. All lines
Last updated on: 07-01-2020 - 15:23
Contact: Hilde Van Esch
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, History of use, Published in peer reviewed journal
Identifying drug-target interactions is a crucial step in drug repositioning, the process of suggesting new indications for known drugs. There are about 9000 FDA-approved and experimental small molecule drugs and more than 500.000 protein records available. Performing in vitro experiments would be
Last updated on: 06-01-2020 - 17:04
Contact: Daniele Parisi
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Biotech/TU-Dresden, Max-Planck-Institut für Informatik Saarbrucken
Status: Still in development
The BALB/c-derived - 4T1 mammary tumor cell line and RAW264.7 macrophage cell line were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin in culture flasks. Harvesting of cultured
Last updated on: 26-11-2019 - 13:12
Contact: Kristel Demeyere
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
This silicone rat is designed for training persons to perform oral gavage of fluids and for learning how to perform blood sampling from the lateral tail vein.
Last updated on: 13-11-2019 - 09:22
FIBEr (Flanders Institute for Biomechanical Experimentation) is a laboratory that investigates the mechanical properties (stiffness, strength, microstructure) of biological tissue, in casu bones, muscles, ligaments, blood vessels, etc. A quantitative characterization of the mechanical behavior of
Last updated on: 12-11-2019 - 11:45
Contact: Nele Famaey
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Still in development, History of use, Internally validated
