A patient-derived explant culture of human prostate cancer to test drug efficacy

Scope of the method

Method relates to
  • Human health
Method is situated in
  • Basic Research
  • Translational - Applied Research
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
Human prostate cancer tissue

Description

Method keywords
  • Explant
  • human
  • Patient-derived
Scientific area keywords
  • prostate
  • cancer
  • drug testing
Method description

Patient-derived explants of prostate cancer provide an ex vivo model that retains the architecture and microenvironment of the native tissue. It enables the evaluation of drug responses on individual patient’s tumors ex vivo without passaging in animals. It is compatible with all molecular analysis methods.

See in the references "A patient-derived explant (PDE) model of hormone-dependent cancer."

Lab equipment
  • CO2-incubator ; 
  • Laminar flow ; 
  • Cell culture room.
Method status
  • History of use
  • Published in peer reviewed journal

Pros, cons & Future potential

Advantages
  • Patient-derived ;
  • Ex vivo ;
  • No animals involved ;
  • Retains original tissue architecture and tumor microenvironment ;
  • Economic ;
  • Reasonable throughput.
Challenges
  • Long-term culture is challenging ;
  • Limited around of material.

References, associated documents and other information

References

A patient-derived explant (PDE) model of hormone-dependent cancer. Centenera MM, Hickey TE, Jindal S, Ryan NK, Ravindranathan P, Mohammed H, Robinson JL, Schiewer MJ, Ma S, Kapur P, Sutherland PD, Hoffmann CE, Roehrborn CG, Gomella LG, Carroll JS, Birrell SN, Knudsen KE, Raj GV, Butler LM, Tilley WD. Mol Oncol. 2018 Sep;12(9):1608-1622. doi: 10.1002/1878-0261.12354. Epub 2018 Aug 16

Associated documents

Contact person

Johan Swinnen

Organisations

KU Leuven
Oncology
Belgium
Flemish Region

Partners

University of Adelaide