We have successfully set-up the iPSC technology and are able to derive human cortical neurons for the study of neurodevelopmental disorders e.g. the MECP2 duplication syndrome (published) and other projects in the lab (ongoing). We also create isogenic lines using CrispR-Cas technology. All lines
Last updated on: 07-01-2020 - 15:23
Contact: Hilde Van Esch
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, History of use, Published in peer reviewed journal
Identifying drug-target interactions is a crucial step in drug repositioning, the process of suggesting new indications for known drugs. There are about 9000 FDA-approved and experimental small molecule drugs and more than 500.000 protein records available. Performing in vitro experiments would be
Last updated on: 06-01-2020 - 17:04
Contact: Daniele Parisi
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Biotech/TU-Dresden, Max-Planck-Institut für Informatik Saarbrucken
Status: Still in development
In view of safety of pregnant women, a promising in vitro zebrafish embryo developmental toxicity assay has been developed to test pharmaceutical and chemical compounds for their teratogenic potential. The protocol deals with exposing zebrafish embryos to a range of compound concentrations at 28°C
Last updated on: 06-01-2020 - 16:55
Contact: Steven Van Cruchten
Organisation: University of Antwerp (UAntwerpen)
Status: Still in development, Published in peer reviewed journal
The ex-vivo endothelium-stent contact model allows measuring the impact of implants on the arterial tissue. The endothelium is directly exposed by inserting a wire made of the investigated material into the lumen of the aortic ring to mimic the contact between the coronary stent and the endothelium.
Last updated on: 03-12-2019 - 17:23
Contact: Eleonora Scarcello
Organisation: Université Catholique de Louvain (UCL)
Partners: Université Catholique de Louvain (UCL)
Status: Internally validated
The cytokinesis-block micronucleus method allows assessing the presence of DNA damage at the chromosome level. It is an essential part of toxicology, because mutation is a crucial event in carcinogenesis. The capacity of inhaled particles to induce irreversible mutations in type II lung epithelial
Last updated on: 26-11-2019 - 17:35
Contact: Violaine Sironval
Organisation: Université Catholique de Louvain (UCL)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
Cell Culture and Reagents hiPSC-CMs can be obtained commercially either as living pre-plated cells seeded onto fibronectin-coated 96-well mClear plates (Greiner Bio-One, No. 655090) or can be plated in house at a density (~25,000 cells/well) suited to forming a confluent synchronously beating mono
Last updated on: 26-11-2019 - 13:17
Contact: Ard Teisman
Organisation: Janssen Pharma of JNJ
Status: Internally validated, Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The BALB/c-derived - 4T1 mammary tumor cell line and RAW264.7 macrophage cell line were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin in culture flasks. Harvesting of cultured
Last updated on: 26-11-2019 - 13:12
Contact: Kristel Demeyere
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The hemolysis assay remarkably predicts the inflammatory potential of inhaled particles. The capacity of particles to damage cellular membranes is a key property to predict their inflammatory potential upon inhalation. In macrophages and epithelial cells exposed to particles, alteration of the
Last updated on: 15-11-2019 - 16:15
Contact: Dominique Lison
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
TEM, NTA and western blot were used to characterize the purified inflammation‐associated EV subpopulations from TNF-α treated HUVEC based on their sedimentation speeds (10K and110K) and surface markers (CDs and ICAM-1). Protein arrays were used to discover the immunomodulatory content of
Last updated on: 14-11-2019 - 14:02
Contact: Baharak Hosseinkhani
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
FIBEr (Flanders Institute for Biomechanical Experimentation) is a laboratory that investigates the mechanical properties (stiffness, strength, microstructure) of biological tissue, in casu bones, muscles, ligaments, blood vessels, etc. A quantitative characterization of the mechanical behavior of
Last updated on: 12-11-2019 - 11:45
Contact: Nele Famaey
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Still in development, History of use, Internally validated
