Hemolysis assay to predict the inflammatory activity of inhaled particles
Scope of the method
- Human health
- Basic Research
- In vitro - Ex vivo
- Human derived cells / tissues / organs
- red blood cells
- inhaled particles
- lung toxicity
The hemolysis assay remarkably predicts the inflammatory potential of inhaled particles. The capacity of particles to damage cellular membranes is a key property to predict their inflammatory potential upon inhalation. In macrophages and epithelial cells exposed to particles, alteration of the phagolysosome membrane is a key event for the activation of the inflammasome and the release of interleukin-1beta. The membranolytic activity of particles can easily be assessed after incubation with red blood cells and measurement of the level of hemoglobin release. This assay can be performed with human red blood cells.
- History of use
- Internally validated
- Published in peer reviewed journal
Pros, cons & Future potential
- Easy ;
- Cheap ;
- Great predictivity.
This assay is mainly applicable to particles that are phagocytozed (low solubility).
References, associated documents and other information
Contact personDominique Lison
Louvain centre for Toxicology and Applied Pharmacology