Our lipidomics profiling and fingerprinting methodology enables the analysis of all lipids within a biological matrix. These lipids can then be compared between an individual or condition with a specific metabolic state and healthy controls to find biomarkers or pathways that can be related to
Last updated on: 08-11-2019 - 14:12
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: History of use, Internally validated, Published in peer reviewed journal
VITO can offer expertise in animal-free methods for inhalation testing. VITO has an Air-Liquid Interface (ALI) platform with three ALI exposure modules: two commercial systems for bronchial studies and one in-house developed system for lower airway studies. At VITO ALI exposure modules, aerosol
Last updated on: 08-11-2019 - 14:08
Contact: Sandra Verstraelen
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Partners: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: History of use, Internally validated, Published in peer reviewed journal
Cells are treated with compounds and after a specific incubation time the MTT reagent is added. In living cells, the MTT is reduced to the purple formazan. The amount of formazan is related to the amount of living cells. Next, the medium and MTT reagent are aspirated and DMSO is added after which
Last updated on: 08-11-2019 - 14:06
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Molecules are descripted by hundreds to thousands different descriptors (e.g. molecular weight, pI, log P,...) after which they can be clustered in different classes.
Last updated on: 08-11-2019 - 14:05
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Transport kinetics accross the skin and/or mucosa is investigated using Franz diffusion cells and LC-UV/MS detection of the investigated molecule in the receptor compartment.
Last updated on: 08-11-2019 - 14:05
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
This work will be based on data describing the rat hindlimb kinematics obtained by X-Ray Reconstruction of Moving Morphology (XROMM) and ground reaction forces following destabilization of the medial meniscus (DMM) and running. These data will be analyzed with multi-body dynamics to estimate the
Last updated on: 08-11-2019 - 14:02
Contact: Judith Piet
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development
A compound of interest (e.g. new psychoactive substance, endocrine disrupting compound, ...) is incubated with human liver microsomes and liver cytosolic fractions to generate both Phase I and II metabolites. Samples are prepared for analysis using a simple method in order to avoid possible losses
Last updated on: 08-11-2019 - 14:01
Contact: Philippe Vervliet
Organisation: University of Antwerp (UAntwerpen)
Status: Published in peer reviewed journal
Three-dimensional (3D) lung epithelial cell models mimic key aspects of the parental tissue, including apical-basolateral polarity and barrier function (Barrila et al. 2010, PMID: 20948552). These 3D cultures are generated using the rotating wall vessel (RWV) bioreactor system, allowing host cells
Last updated on: 08-11-2019 - 13:59
Contact: Aurélie Crabbé
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The Galleria mellonella assay makes use of larvae of the Greater Wax Moth. G. mellonella larvae are injected with a small amount of microorganisms, and survival can easily be followed for up to 1 week. This allows to compare virulence between different organisms. Alternatively, after injection of
Last updated on: 08-11-2019 - 13:58
Contact: Tom Coenye
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The C. elegans assay can be used to study the virulence of various microorganisms, and can be used to evaluate the antimicrobial effect of drugs or drug candidates. The two main outcome parameters are (i) survival of C. elegans and (ii) microbial load.
Last updated on: 08-11-2019 - 13:57
Contact: Tom Coenye
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
