Amoebae are natural eukaryotic predators of bacteria, yeasts, fungi and they are ubiquiste. They are excellent and easy-to-use cellular infection models, as they allow to co-cultivate any organisms in a broad range of infection medium, compatible with high quality microscopy techniques,
Last updated on: 16-03-2022 - 14:45
Contact: Charles Van der Henst
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
A calculated number of bacteria is injected through the pro-legs of the larvae. As several entry points are present, this allows to inject virulent bacteria and potential antimicrobial compounds within the same host. The survival of the larvae is scored over time (days post inoculation) until about
Last updated on: 16-03-2022 - 14:43
Contact: Charles Van der Henst
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
Follicle Biology Laboratory has developed a well characterized and standardized MOUSE in vitro follicle culture (IFC) system. In this system, early stage ovarian follicles are cultured in vitro under physiological hormone concentrations up to fertilizable and developmentally competent mature oocytes
Last updated on: 16-03-2022 - 14:32
Contact: Ellen Anckaert
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
Prototyping and replication (small series production) of microfluidic or optofluidic devices, in thermoplastic polymers or in glass. 3D nanoprinting is also available to produce microscaffolds, possibly within microfluidic channels.
Last updated on: 16-03-2022 - 14:25
Contact: Jürgen Van Erps
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
Recent improvements on the structural aspects of organ-on-chips pave the way towards a large-scale application. As such soon the number of read-out instruments that are in operation in parallel will need to drastically increase. Unfortunately, standard read-out equipment is bulky, complex and
Last updated on: 16-03-2022 - 14:23
Contact: Wendy Meulebroeck
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
We developed a high-throughput live-cell imaging-based organoid analysis platform, Organoid Brightfield Identification-based Therapy Screening (Orbits), that allows for kinetic monitoring of organoid growth and drug responses in routine extracellular matrix domes, high-throughput 384-well
Last updated on: 16-03-2022 - 13:59
Contact: Christophe Deben
Organisation: University of Antwerp (UAntwerpen)
Status: Internally validated
Quantitative Structure Activity Relationship modeling is generally used to construct models in which molecular descriptors of chemical compounds are used to predict endpoints/activities of interest. Commercial packages are available that can be implemented, but new models can be constructed if
Last updated on: 16-03-2022 - 13:49
Contact: Geert Verheyen
Organisation: Thomas More University of Applied Sciences
Status: Still in development, History of use, Published in peer reviewed journal
The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration
Last updated on: 28-02-2022 - 11:04
Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
HeLa cells are the first continuous cancer cell line and were isolated from the aggressive glandular cervical cancer of a 31-year old woman. It was the first aneuploid line derived from human tissue maintained in continuous cell culture. Knowledge of almost every process that occurs in human cells
Last updated on: 28-02-2022 - 11:01
Contact: Jessie Neuckermans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development, History of use
Using this method you can measure the (relative) lipid load in human skin-derived stem cells differentiated towards hepatic cells. This method could also be applied on other cell types (e.g. HepG2), since it is based on the following publication: "M. T. Donato et al., Chem. Biol. Interact. 181, 417
Last updated on: 28-02-2022 - 11:00
Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated
