These are the general guidelines on sample input requirements to successfully start our RIBO-seq procedures:
Cell lines in suspension: pellet of 10 – 50 M snap frozen cells. The pre-treatment and collection specifications are also mentioned in our pre-processing protocol.
Adherent cell lines
Last updated on: 21-10-2022 - 10:33
Contact: Zarel Martinez
Organisation: OHMX.bio
Status: History of use, Internally validated, Published in peer reviewed journal
The in vitro rumen fermentation technique is used to simulate the rumen function/fermentation. The rumen is the first compartment of the digestive tract of ruminants, in which feed - consumed by ruminants - is fermented by rumen microbes. For optimal fermentation, the rumen provides an anaerobic
Last updated on: 14-10-2022 - 11:37
Contact: Veerle Fievez
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The tri-dimensional (3D) culture protocols allow isolation and culture of gastric epithelial antral and intestinal stem cells to efficiently generate organoids that recapitulate the mature pyloric and intestinal epithelium in vitro and foetal epithelial progenitors of these tissues growing as
Last updated on: 16-08-2022 - 10:57
Contact: Marie-Isabelle Garcia
Organisation: Université Libre de Bruxelles (ULB)
Status: History of use, Internally validated, Published in peer reviewed journal
For therapeutic or reporter molecules to be effective for therapy or imaging applications, proper accumulation of the compounds in the tissue of interest is required, with minimal accumulation in undesired organs to avoid toxic side-effects and increase bioavailability. After initial in vitro
Last updated on: 09-08-2022 - 16:34
The main objective of this research project is to create the first full-thickness cornea-on-chip, which comprises a 3D construct with every cellular layer of the cornea. This construct is embedded in a microfluidic chip with two channels that are continuously perfused. The epithelial side of the
Last updated on: 27-07-2022 - 09:35
Contact: Joris Van Meenen
Organisation: University of Antwerp (UAntwerpen)
Status: Still in development
In vitro differentiation of hematopoietic stem cells (HSC) or inducible pluripotent stem cells (IPS) to megakaryocytes and platelets using specific differentiation conditions (liquid and 3D media). CRISPR/cas mutagenesis of HSC or IPS to study the effect of gene depletion or specific mutants on
Last updated on: 26-05-2022 - 10:41
Contact: Kathleen Freson
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Internally validated, Published in peer reviewed journal
The tetanus neurotoxin (TeNT) is one of the most toxic proteins known to man, which prior to the use of the vaccine against the TeNT producing bacteria Clostridium tetani, resulted in a 20% mortality rate upon infection. The clinical detrimental effects of tetanus have decreased immensely since the
Last updated on: 22-03-2022 - 16:49
Lung cancer thrives in a complex multicellular tumor microenvironment (TME) that impacts tumor growth, metastasis, response, and resistance to therapy. While orthotopic murine lung cancer models can partly recapitulate this complexity, they do not resonate with high-throughput immunotherapeutic drug
Last updated on: 16-03-2022 - 14:53
Contact: Cleo Goyvaerts
Organisation: Vrije Universiteit Brussel (VUB)
Partners: Institut Curie
Status: Published in peer reviewed journal
This model is a 3D human co-culture model where both hepatocyte functionality and HSC quiescence can be maintained for at least 21 days. This novel system allows hepatotoxicity testing and can detect drug-induced as well as hepatocyte-dependent HSC activation, thereby representing an important step
Last updated on: 16-03-2022 - 14:51
Generation of Hepatic Stellate Cells from Human Pluripotent Stem for in vitro liver fibrosis studies
We established a protocol to efficiently generate hepatic stellate cells (HSCs) from human pluripotent stem cells (PSCs). Our procedure generated complex in vitro spheroid cultures that better mimic the complexity of the liver as well as liver function. In co-culture, iPSC-HSCs promote maintenance
Last updated on: 16-03-2022 - 14:49
Contact: Leo A. van Grunsven
Organisation: Vrije Universiteit Brussel (VUB)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
