The hemolysis assay remarkably predicts the inflammatory potential of inhaled particles. The capacity of particles to damage cellular membranes is a key property to predict their inflammatory potential upon inhalation. In macrophages and epithelial cells exposed to particles, alteration of the
Last updated on: 15-11-2019 - 16:15
Contact: Dominique Lison
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
TEM, NTA and western blot were used to characterize the purified inflammation‐associated EV subpopulations from TNF-α treated HUVEC based on their sedimentation speeds (10K and110K) and surface markers (CDs and ICAM-1). Protein arrays were used to discover the immunomodulatory content of
Last updated on: 14-11-2019 - 14:02
Contact: Baharak Hosseinkhani
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
FIBEr (Flanders Institute for Biomechanical Experimentation) is a laboratory that investigates the mechanical properties (stiffness, strength, microstructure) of biological tissue, in casu bones, muscles, ligaments, blood vessels, etc. A quantitative characterization of the mechanical behavior of
Last updated on: 12-11-2019 - 11:45
Contact: Nele Famaey
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Still in development, History of use, Internally validated
Integrated approach for the characterization,validation and monitoring of protein biomarkers in animal tissue samples as well as on human tissue samples. The ''cell-block'' technique allows the study of cell lines with the same approach. The methodology involves histological and standardized
Last updated on: 12-11-2019 - 11:37
Contact: Cédric Balsat
Organisation: Université Libre de Bruxelles (ULB)
Partners: Université Libre de Bruxelles (ULB)
Status: Published in peer reviewed journal
Epigenetics have taken centre stage in the study of diseases such as cancer, diabetes, and neurodegeneration. A new field is emerging that targets epigenetic modifications for therapeutic intervention: pharmacoepigenetics. Particularly in oncology, inhibitors of epigenetic-modifying proteins, i.e.
Last updated on: 12-11-2019 - 11:34
Contact: Maarten Dhaenens
Organisation: Ghent University (UGent)
Status: Still in development, Internally validated, Published in peer reviewed journal
It is the goal of the DNA-adductomics to search for DNA-adducts that might be formed during interaction with contaminants. The analysis of DNA adducts is performed using ultra-high performance liquid chromatography coupled to hybrid quadrupole-Orbitrap high resolution mass spectrometry. Both the
Last updated on: 12-11-2019 - 11:16
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
Our polar metabolomics profiling and fingerprinting methodology applies ultra-high performance liquid chromatography coupled to hybrid quadrupole-Orbitrap high resolution mass spectrometry. Both the instrumental method, as well as generic extraction protocols for colon tissue, cell cultures, urine,
Last updated on: 12-11-2019 - 11:13
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
The aim of these in vitro digestions is to simulate the gastrointestinal digestion of specific food sources and to identify metabolites that might be formed out of this food source by the residing microbiome. For this purpose, fecal samples will be collected from volunteers and will be prepared as
Last updated on: 12-11-2019 - 10:56
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Our lipidomics profiling and fingerprinting methodology enables the analysis of all lipids within a biological matrix. These lipids can then be compared between an individual or condition with a specific metabolic state and healthy controls to find biomarkers or pathways that can be related to
Last updated on: 08-11-2019 - 14:12
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: History of use, Internally validated, Published in peer reviewed journal
VITO can offer expertise in animal-free methods for inhalation testing. VITO has an Air-Liquid Interface (ALI) platform with three ALI exposure modules: two commercial systems for bronchial studies and one in-house developed system for lower airway studies. At VITO ALI exposure modules, aerosol
Last updated on: 08-11-2019 - 14:08
Contact: Sandra Verstraelen
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Partners: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: History of use, Internally validated, Published in peer reviewed journal
