The neutral red uptake (NRU) assay provides a quantitative measurement of the number of viable cells. The test is based on the ability of living cells to take up and bind neutral red (NR), a dye which easily penetrates cell membranes via non-ionic diffusion and accumulates in the lysosomes. Dying
Last updated on: 21-02-2022 - 15:23
Contact: Roel Anthonissen
Organisation: Sciensano
Status: History of use, Published in peer reviewed journal
The in vitro Alkaline Comet Assay is a microgel electrophoresis technique which allows to measure DNA damage (single and double strand breaks, alkali labile sites, incomplete excision repair sites and cross links) cell by cell. Cells are mixed with 0.8% Low Melting Point Agarose which is spread as a
Last updated on: 21-02-2022 - 15:21
Contact: Roel Anthonissen
Organisation: Sciensano
Status: History of use, Published in peer reviewed journal
The Ames test is a short-term bacterial reverse gene mutation assay specifically designed to detect a wide range of chemical substances that can produce genetic damage that leads to permanent gene mutations. The test has been described in detail in OECD TG 471 and employs several histidine dependent
Last updated on: 21-02-2022 - 15:15
Contact: Roel Anthonissen
Organisation: Sciensano
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
Alkaline comet assay (COM) and Micronucleus test (MN) are well-established cytogenetic tests that are used to detect both immediate damages i.e. DNA fragmentation, and permanent damage which results in micronuclei formation. These techniques are often applied separately or, sometimes, in combination
Last updated on: 18-02-2022 - 16:42
Contact: Ha Nguyen
Organisation: Université Libre de Bruxelles (ULB), Sciensano
Partners: University of Liège (ULiège)
Status: History of use, Internally validated
We aimed to optimize fibrin matrix composition in order to mimic human ovarian tissue architecture for human ovarian follicle encapsulation and grafting. Ultrastructure of fresh human ovarian cortex in age-related women (n = 3) and different fibrin formulations (F12.5/T1, F30/T50, F50/T50, F75/T75),
Last updated on: 10-09-2021 - 14:18
Contact: Christiani Amorim
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
We developed an innovative model to study the effect of the supernumerary X chromosome on KS features. The model was generated using induced pluripotent stem cells (iPSCs) from patients with Klinefelter syndrome (KS) i.e. with a 47, XXY karyotype. In order to compare the potentials of both 47XXY
Last updated on: 07-01-2021 - 21:23
Contact: Christine Wyns
Organisation: Université Catholique de Louvain (UCL)
Partners: Geneva University Hospitals
Status: Published in peer reviewed journal
The ex-vivo endothelium-stent contact model allows measuring the impact of implants on the arterial tissue. The endothelium is directly exposed by inserting a wire made of the investigated material into the lumen of the aortic ring to mimic the contact between the coronary stent and the endothelium.
Last updated on: 03-12-2019 - 17:23
Contact: Eleonora Scarcello
Organisation: Université Catholique de Louvain (UCL)
Partners: Université Catholique de Louvain (UCL)
Status: Internally validated
The cytokinesis-block micronucleus method allows assessing the presence of DNA damage at the chromosome level. It is an essential part of toxicology, because mutation is a crucial event in carcinogenesis. The capacity of inhaled particles to induce irreversible mutations in type II lung epithelial
Last updated on: 26-11-2019 - 17:35
Contact: Violaine Sironval
Organisation: Université Catholique de Louvain (UCL)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
The hemolysis assay remarkably predicts the inflammatory potential of inhaled particles. The capacity of particles to damage cellular membranes is a key property to predict their inflammatory potential upon inhalation. In macrophages and epithelial cells exposed to particles, alteration of the
Last updated on: 15-11-2019 - 16:15
Contact: Dominique Lison
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
Hyper Polarized (HP) MR allows to considerably increase the sensitivity (>10.000) of MR spectroscopy and spectroscopic imaging. The hyperpolarized molecule can be injected at room temperature to a cell system or an in vivo model and its metabolism can be followed over a few minutes.The technique
Last updated on: 08-11-2019 - 13:56
Contact: Bénédicte Jordan
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
