Our lipidomics profiling and fingerprinting methodology enables the analysis of all lipids within a biological matrix. These lipids can then be compared between an individual or condition with a specific metabolic state and healthy controls to find biomarkers or pathways that can be related to
Last updated on: 08-11-2019 - 14:12
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: History of use, Internally validated, Published in peer reviewed journal
Cells are treated with compounds and after a specific incubation time the MTT reagent is added. In living cells, the MTT is reduced to the purple formazan. The amount of formazan is related to the amount of living cells. Next, the medium and MTT reagent are aspirated and DMSO is added after which
Last updated on: 08-11-2019 - 14:06
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Molecules are descripted by hundreds to thousands different descriptors (e.g. molecular weight, pI, log P,...) after which they can be clustered in different classes.
Last updated on: 08-11-2019 - 14:05
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Transport kinetics accross the skin and/or mucosa is investigated using Franz diffusion cells and LC-UV/MS detection of the investigated molecule in the receptor compartment.
Last updated on: 08-11-2019 - 14:05
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
Three-dimensional (3D) lung epithelial cell models mimic key aspects of the parental tissue, including apical-basolateral polarity and barrier function (Barrila et al. 2010, PMID: 20948552). These 3D cultures are generated using the rotating wall vessel (RWV) bioreactor system, allowing host cells
Last updated on: 08-11-2019 - 13:59
Contact: Aurélie Crabbé
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The Galleria mellonella assay makes use of larvae of the Greater Wax Moth. G. mellonella larvae are injected with a small amount of microorganisms, and survival can easily be followed for up to 1 week. This allows to compare virulence between different organisms. Alternatively, after injection of
Last updated on: 08-11-2019 - 13:58
Contact: Tom Coenye
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The C. elegans assay can be used to study the virulence of various microorganisms, and can be used to evaluate the antimicrobial effect of drugs or drug candidates. The two main outcome parameters are (i) survival of C. elegans and (ii) microbial load.
Last updated on: 08-11-2019 - 13:57
Contact: Tom Coenye
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
This method evaluates sprouting propensity of endothelial cells in a collagen matrix. The effect of pro-angiogenic growth factors or co-cultured cells can be measured by quantifying the amount of vascular sprouts that form on endothelial spheroids. Endothelial spheroids are obtained by growing
Last updated on: 08-11-2019 - 13:47
The chorioallantoic membrane (CAM) is a highly vascularized membrane which results from the fusion of two extra-embryonic membranes, namely the chorion and the allantois. The CAM is easily accessible for manipulation and imaging, but methodologies differ whether the chicken embryo stays within its
Last updated on: 08-11-2019 - 13:27
Better understanding of molecular mechanisms controlling both normal and malignant human hematopoiesis will lead to a more efficient therapy of immune deficiencies and lymphoid leukemias. Therefore, human hematopoietic progenitor cells (HPCs) are differentiated in vitro towards distinct
Last updated on: 08-11-2019 - 13:21
Contact: Tom Taghon
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
