Isolation Femoral and tibial bones are isolated from 10-12 weeks old C57BL/6J mice. After cutting the bone's ends, the bone marrow is flushed out using a 23G syringe with PBS into a 50ml falcon tube. After centrifugation cells are re-suspended in FCS (0.5ml/mouse leg). Differentiation 0.5ml of the
Last updated on: 14-08-2020 - 15:26
Contact: Jerome Hendriks
Organisation: University of Hasselt (UHasselt)
Status: History of use, Internally validated, Published in peer reviewed journal
Characterization of Human Oligodendroglia derived-EV-rich in sHSPs (HOG-EV-sHSPs). Establish HSPB1/B8 stably expressing cell lines for the production of sHSPs rich-EV cell culture conditioned medium. Human Oligodendroglia cell lines (HOG) were stably transduced with pLenti-HSPBs gene constructs
Last updated on: 14-08-2020 - 15:22
Contact: Joy Irobi
Organisation: University of Hasselt (UHasselt)
Status: Still in development, Internally validated, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
Peripheral blood mononuclear cells (PBMC) are isolated from the peripheral blood using Ficoll gradient centrifugation. The PBMC are then stained with fluorescently-labelled monoclonal antibodies directed against cell surface or intracellular proteins that can be used to identify specific immune cell
Last updated on: 03-03-2020 - 11:46
Contact: Cannot be disclosed
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal
Cell line which is commonly used to study biological processes in mouse macrophages. It is a macrophage-like cell line derived from BALB/c mice.
Last updated on: 25-02-2020 - 23:58
The ex-vivo endothelium-stent contact model allows measuring the impact of implants on the arterial tissue. The endothelium is directly exposed by inserting a wire made of the investigated material into the lumen of the aortic ring to mimic the contact between the coronary stent and the endothelium.
Last updated on: 03-12-2019 - 17:23
Contact: Eleonora Scarcello
Organisation: Université Catholique de Louvain (UCL)
Partners: Université Catholique de Louvain (UCL)
Status: Internally validated
The cytokinesis-block micronucleus method allows assessing the presence of DNA damage at the chromosome level. It is an essential part of toxicology, because mutation is a crucial event in carcinogenesis. The capacity of inhaled particles to induce irreversible mutations in type II lung epithelial
Last updated on: 26-11-2019 - 17:35
Contact: Violaine Sironval
Organisation: Université Catholique de Louvain (UCL)
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
Worldwide, outdoor air pollution is responsible for 4.2 million premature deaths per year. Both chronic and acute exposure to particulate matter air pollution is a risk factor for heart and lung diseases. One of the atmospheric pollutant particles is represented by soot or black carbon (BC)
Last updated on: 15-11-2019 - 16:16
Contact: Eva Bongaerts
Organisation: University of Hasselt (UHasselt)
Partners: University of Hasselt (UHasselt), Katholieke Universiteit Leuven (KUL)
Status: History of use, Internally validated, Published in peer reviewed journal
The hemolysis assay remarkably predicts the inflammatory potential of inhaled particles. The capacity of particles to damage cellular membranes is a key property to predict their inflammatory potential upon inhalation. In macrophages and epithelial cells exposed to particles, alteration of the
Last updated on: 15-11-2019 - 16:15
Contact: Dominique Lison
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
TEM, NTA and western blot were used to characterize the purified inflammation‐associated EV subpopulations from TNF-α treated HUVEC based on their sedimentation speeds (10K and110K) and surface markers (CDs and ICAM-1). Protein arrays were used to discover the immunomodulatory content of
Last updated on: 14-11-2019 - 14:02
Contact: Baharak Hosseinkhani
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
Hyper Polarized (HP) MR allows to considerably increase the sensitivity (>10.000) of MR spectroscopy and spectroscopic imaging. The hyperpolarized molecule can be injected at room temperature to a cell system or an in vivo model and its metabolism can be followed over a few minutes.The technique
Last updated on: 08-11-2019 - 13:56
Contact: Bénédicte Jordan
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
