Inflammatory extracellular vesicles
Scope of the method
The Method relates to
- Human health
The Method is situated in
- Basic Research
- Translational - Applied Research
Type of method
- In vitro - Ex vivo
This method makes use of
- Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
HUVEC/ THP-1
Description
Method keywords
- EV isolation
- EV charactrization
- profiling
Scientific area keywords
- cardiovascular disorders
Method description
TEM, NTA and western blot were used to characterize the purified inflammation‐associated EV subpopulations from TNF-α treated HUVEC based on their sedimentation speeds (10K and110K) and surface markers (CDs and ICAM-1). Protein arrays were used to discover the immunomodulatory content of subpopulations. In addition, functional integrity of the EV subpopulations was assessed using migration cell based assays.
Lab equipment
- NTA
- TEM
- WB
- Protein array
- SPR
- FACs
Method status
- Published in peer reviewed journal
- Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
Contact person
Baharak HosseinkhaniOrganisations
University of Hasselt (UHasselt)Bionanotechnology Research Group, Biomedical Research Institute (BIOMED), Hasselt University, Martelarenlaan 42, B-3500 Hasselt, Belgium
Belgium
Flemish Region