The extracellular matrix (ECM) is a highly organized tissue-specific network, which is secreted by tissue-resident cells and significantly influences cell phenotype, behaviour and function. The 3R principle calls for the replacement of animal models in areas like drug screening, and consequently,
Last updated on: 13-08-2024 - 09:57
Overuse tendon injuries are a major cause of musculoskeletal morbidity in both human and equine athletes. Despite the big burden, there is no effective treatment to restore tendon’s natural composition, due to a lack of understanding of fundamental cell biology. Additionally, translation of novel
Last updated on: 12-08-2024 - 09:32
The use of three-dimensional (3D) cell culture models is considered a reliable model that is successfully used for high-throughput drug testing in carcinoma research. However, the development of soft tissue sarcoma organoids is lagging behind. Soft tissue sarcoma is a group of rare malignancies from
Last updated on: 30-07-2024 - 12:08
Contact: Agnieszka Wozniak
Organisation: Oncology - KU Leuven, Katholieke Universiteit Leuven (KUL)
Status: Still in development
Using the combination of advanced CRIPSR tools including several Cas orthologs, based editors (ABE, CBE, CGBE) and prime editing technologie with efficient delivery vehicles such as LV, AAV and virus-like particles (VLPs), our lab has become experienced with introducing or replacing precise edits in
Last updated on: 21-06-2024 - 11:29
Contact: Marianne Carlon
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Internally validated
The DETECTOR algorithm allows to functionally screen genetic strategies for Cystic Fibrosis in patient-derived rectal organoids. DETECTOR is a machine-learning based software that takes frames from forskolin-induced swelling (FIS) assay on organoids as input and gives the number of functionally
Last updated on: 10-06-2024 - 11:17
Contact: Marianne Carlon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Adult intestinal stem cells are grown into closed 3D structures with protruding buds and a luminal compartment. These 3D structures are produced from the stem cells present in the base of crypts isolated from rectal mucosa samples that can be collected by suction or forceps biopsy. The crypts are
Last updated on: 22-02-2024 - 11:15
Contact: Anabela Ramalho
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The application of liver organoids is very promising in the field of liver tissue engineering; however, it is still facing some limitations. One of the current major limitations is the matrix in which they are cultured. The mainly undefined and murine-originated tumor matrices derived from
Last updated on: 22-01-2024 - 17:28
Contact: Nathan Carpentier
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
In contrast to human medicine, standard imaging techniques for cardiac interventions, namely fluoroscopy, CT and MRI, cannot be used in horses because the size of their thorax is too large for the gantry (CT and MRI) or results in insufficient detail (fluoroscopy). Therefore, echocardiography is
Last updated on: 20-09-2023 - 10:28
Safety Assessment of Compounds after In Vitro Metabolic Conversion Using Zebrafish Eleuthero Embryos
Zebrafish-based platforms have recently emerged as a useful tool for toxicity testing as they combine the advantages of in vitro and in vivo methodologies. Nevertheless, the capacity to metabolically convert xenobiotics by zebrafish eleuthero embryos is supposedly low. To circumvent this concern, a
Last updated on: 01-09-2023 - 09:55
Contact: Marie Pardon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The spatiotemporal distribution of fluorescent compounds was examined during 48 h after immersion (10 µM) or microinjection (2 mg/kg) in the pericardial cavity (PC), intraperitoneally (IP) and yolk sac (IY) of 3 dpf zebrafsh eleuthero-embryos. By modelling the fluorescence of whole-body contours
Last updated on: 01-09-2023 - 09:43
Contact: Marie Pardon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
