Human choroid plexus organoids

Commonly used acronym: ChP organoids

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Scope of the method

The Method relates to
  • Human health
The Method is situated in
  • Basic Research
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
Induced pluripotent stemcells / embryonic stemcells

Description

Method keywords
  • matrigel
  • human embryonic stem cell derived organoid model
  • Human induced Pluripotent Stem Cell
  • HUman brain organoids
Scientific area keywords
  • choroid plexus
  • blood-csf barrier
  • brain delivery
  • csf
Method description
ChP organoids are produced starting from human induced pluripotent or embryonic stem cells. 7 days after seeding of the stem cells, the organoids are embedded in matrigel. After 30-40 days, the organoids are differentiated and form a functional blood-CSF barrier with fluid-filled cysts resembling human CSF. This method has first been described and published by Laura Pellegrini (2020).
Lab equipment
  • - Shaker in incubator with 5% CO2 at 37°C,
  • - General cell culture equipment and flows.
Method status
  • Published in peer reviewed journal

Pros, cons & Future potential

Advantages

This system can be more relevant to study the human choroid plexus and CSF in comparison to the use of other in vitro models with immortalized cells. The organoids produce a CSF-like fluid, enabling study of CSF production and composition in a much less invasive and difficult way compared to the use of mice.

Challenges

The long culturing time is a downside. Additionally, there might be variability between different batches, influence of the batch of matrigel or other components used during culturing, and there is a big impact of the cell type that is used.

References, associated documents and other information

References
  • Laura Pellegrini et al. ,Human CNS barrier-forming organoids with cerebrospinal fluid production. Science369,eaaz5626(2020). DOI:10.1126/science.aaz5626
  • https://www.stemcell.com/stemdiff-choroid-plexus-organoid-differentiation-kit.html
Associated documents
Publication from Laura Pellegrini.pdf
Protocol ChP organoids from stemcell technologies kit.pdf
Links
Human CNS barrier-forming organoids with cerebrospinal fluid production, L. Pel…

Contact person

Xenia Ghysel

Organisations

Vlaams Instituut voor Biotechnologie (VIB)
Center for Inflammation Research
Barriers in inflammation lab
Belgium
Flemish Region

Ghent University (UGent)
Biomedical molecular biology
Barriers in inflammation lab
Belgium
Flemish Region