Physiologically based pharmacokinetic (PBPK) modeling is an in-silico modelling technique that aims to predict how a drug moves through the body by mathematically representing real anatomical and physiological structures. Overall, PBPK provides a powerful framework for bridging in vitro data,
Last updated on: 18-11-2025 - 14:26
Contact: Pieter-Jan De Sutter
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Gelatin-methacryloyl-aminoethyl-methacrylate (gel-MA-AEMA)-, and gelatin-methacryloyl-norbornene (gel-MA-NB)-based biomaterial inks fabricated into 3D hydrogels (“villi only” versus “crypts and villi”) with digital light processing and co-cultured Caco-2/HT29-MTX cells.
Last updated on: 18-11-2025 - 14:19
Contact: Debby Laukens
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
We established a human multi-organ-on-chip model to recapitulate key physiological elements of gut-brain communication via the systemic circulation and the blood-cerebrospinal fluid barrier (BCSFB). In our model, a gut-blood barrier, comprised of Caco-2 (enterocytes) and HT29-MTX cells (goblet cells
Last updated on: 18-11-2025 - 13:55
Contact: Debby Laukens
Organisation: Ghent University (UGent), VIB - UGent
Status: Still in development
The scope of our research ranges from the study of flow and transport processes in blood and biological fluids in the cardiovascular system and artificial organs to biomechanical aspects of the cardiovascular and the skeleto-muscular system and medical devices. All research tracks explored by our
Last updated on: 17-11-2025 - 12:19
Current pre-clinical drug safety evaluation methods remain costly, inefficient, and unreliable, with 80–90% of compounds ultimately failing in human trials — often due to poor predictive models and safety concerns. Scaffold-based 3D organ models offer a more promising and physiologically relevant
Last updated on: 02-10-2025 - 14:55
Contact: Jolanda van Hengel
Organisation: Ghent University (UGent), Ghent University hospital (UZ Gent), Interuniversitair Micro-Electronica Centrum (IMEC), University of Hasselt (UHasselt)
Status: Still in development
Here, we introduce an open-access Spheroid Light Microscopy Image Atlas (SLiMIA) that can serve as a training set for morphology studies of 3D cell cultures. We provide images with a variety of metadata: 9 microscopes, 47 cell lines, 8 culture media, 4 spheroid formation methods and multiple cell
Last updated on: 01-10-2025 - 08:40
Contact: Eva Blondeel
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Here we present the self-assembly of single-cell suspensions into spheroids by the liquid overlay method, followed by a modular framework for a multifaceted phenotyping of spheroids. Cell seeding, supernatant handling and compound administration are elaborated by both manual and automated procedures
Last updated on: 01-10-2025 - 08:37
Contact: Eva Blondeel
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
ChP organoids are produced starting from human induced pluripotent or embryonic stem cells. 7 days after seeding of the stem cells, the organoids are embedded in matrigel. After 30-40 days, the organoids are differentiated and form a functional blood-CSF barrier with fluid-filled cysts resembling
Last updated on: 08-09-2025 - 12:04
Contact: Xenia Ghysel
Organisation: Vlaams Instituut voor Biotechnologie (VIB), Ghent University (UGent)
Status: Published in peer reviewed journal
The M-SHIME is a dynamic model for the human digestive tract that mimics the different compartments - stomach, small intestine and colon - while also incorporating a cross-sectional simulation mimicking both the luminal as the mucosal microenvironment of the human gut. The mucosal environment in
Last updated on: 04-03-2025 - 13:23
Contact: Tom Van de Wiele
Organisation: Ghent University (UGent)
Status: History of use, Internally validated, Published in peer reviewed journal
The BALB/c-derived - 4T1 mammary tumor cell line and RAW264.7 macrophage cell line were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin in culture flasks. Harvesting of cultured
Last updated on: 28-01-2025 - 16:41
Contact: Kristel Demeyere
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
