Adult skin stem cell-derived in vitro model of hepatic steatosis

Commonly used acronym: Steatosis model

Scope of the method

Alternative method relates to
  • Human health
Alternative method is situated in
  • Translational - Applied Research
Type of alternative method
  • In vitro - Ex vivo
This method makes use of
  • Human derived cells / tissues / organs
Species from which cells/tissues/organs are derived
Human
Type of cells/tissues/organs
Skin-derived adult stem cells
Specify the type of cells/tissues/organs
Human skin-derived hepatic cells

Description

Method keywords
  • Stem cells
  • differentiation
  • Gene expression
  • in vitro
  • Lipids
Scientific area keywords
  • Steatosis
  • liver
  • NAFLD
  • metabolic syndrome
  • lifestyle
  • hepatology
Method description

Human skin-derived adult stem cells differentiated towards hepatic cells (hSKP-HPC) are used in this method (R. M. Rodrigues et al., Stem Cells Dev. 23, 44–55 (2014)). These cells are exposed to a cocktail of insulin and glucose at certain concentrations. After 24h of exposure, these cells exhibit a strong induction of lipogenic genes and accumulate neutral lipids. Using this model, potential new anti-steatotis and anti-non-alcoholic steatohepatitis (NASH) drugs can be tested for their anti-steatotic potentials. The read-outs for this in vitro disease model are (i) gene expression analysis and (ii) neutral lipids quantification.

Lab equipment
  • Biosafety cabinet
  • Flow-cytometer
  • RT-qPCR
  • Cell culture equipment
Method status
  • Still in development

Pros, cons & Future potential

Advantages
  • Fast (24h)
  • Human-relevant
Challenges

Lipid load is only +/- 1.5 -2 x fold higher in the steatosis condition vs the control condition

Modifications

Addition of other sugars

Future & Other applications

The main application is located in preclinical drug testing

References, associated documents and other information

References

R. M. Rodrigues et al., Stem Cells Dev. 23, 44–55 (2014). R. M. Rodrigues et al., Arch. Toxicol. 90, 677–689 (2016)

Associated documents

Contact person

Joost Boeckmans

Organisations

Vrije Universiteit Brussel
Pharmaceutical and Pharmacological Sciences (FARM)
In Vitro Toxicology and Dermato-cosmetology
Belgium