Cerebellar brain slice model

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Scope of the method

The Method relates to

Animal health
Human health

The Method is situated in

Basic Research

Type of method

In vitro - Ex vivo

This method makes use of

Animal derived cells / tissues / organs

Species from which cells/tissues/organs are derived

Mus Musculus

Type of cells/tissues/organs

Brain (cerebellum)

Description

Method keywords

brainslices
cell culture
isolation
mouse

Scientific area keywords

basic research
fundamental research
neuroscience
myelin

Method description

This method describes the steps from a living mouse to a multi-cellular brain slice model where complex cellular interactions can be evaluated.

Method status

Still in development

Pros, cons & Future potential

Advantages

By maintaining brain morphology and ultrastructurally the brain cells present, a complex multicellular system is being formed where the interplay between different cells can be evaluated to identify novel remyelinating therapeutics, targets,…

Challenges

Inter-species differences ;
Terminal experiment for the lab animal ;
Requires a training period due to the susceptibility of the brain slices to cell death.