Our polar metabolomics profiling and fingerprinting methodology applies ultra-high performance liquid chromatography coupled to hybrid quadrupole-Orbitrap high resolution mass spectrometry. Both the instrumental method, as well as generic extraction protocols for colon tissue, cell cultures, urine,
Last updated on: 12-11-2019 - 11:13
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
The aim of these in vitro digestions is to simulate the gastrointestinal digestion of specific food sources and to identify metabolites that might be formed out of this food source by the residing microbiome. For this purpose, fecal samples will be collected from volunteers and will be prepared as
Last updated on: 12-11-2019 - 10:56
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Our lipidomics profiling and fingerprinting methodology enables the analysis of all lipids within a biological matrix. These lipids can then be compared between an individual or condition with a specific metabolic state and healthy controls to find biomarkers or pathways that can be related to
Last updated on: 08-11-2019 - 14:12
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: History of use, Internally validated, Published in peer reviewed journal
Aortic aneurysms consist of a localized dilatation of the aorta resulting from compromised structural integrity and are characterized by a diameter increase of at least 50% compared to a reference diameter. When left untreated, aneurysms tend to progressively enlarge, with an increased risk of
Last updated on: 08-11-2019 - 14:11
Contact: Nele Famaey
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Published in peer reviewed journal
VITO can offer expertise in animal-free methods for inhalation testing. VITO has an Air-Liquid Interface (ALI) platform with three ALI exposure modules: two commercial systems for bronchial studies and one in-house developed system for lower airway studies. At VITO ALI exposure modules, aerosol
Last updated on: 08-11-2019 - 14:08
Contact: Sandra Verstraelen
Organisation: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Partners: Vlaamse Instelling voor Technologisch Onderzoek (VITO)
Status: History of use, Internally validated, Published in peer reviewed journal
Cells are treated with compounds and after a specific incubation time the MTT reagent is added. In living cells, the MTT is reduced to the purple formazan. The amount of formazan is related to the amount of living cells. Next, the medium and MTT reagent are aspirated and DMSO is added after which
Last updated on: 08-11-2019 - 14:06
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Molecules are descripted by hundreds to thousands different descriptors (e.g. molecular weight, pI, log P,...) after which they can be clustered in different classes.
Last updated on: 08-11-2019 - 14:05
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Transport kinetics accross the skin and/or mucosa is investigated using Franz diffusion cells and LC-UV/MS detection of the investigated molecule in the receptor compartment.
Last updated on: 08-11-2019 - 14:05
Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
This work will be based on data describing the rat hindlimb kinematics obtained by X-Ray Reconstruction of Moving Morphology (XROMM) and ground reaction forces following destabilization of the medial meniscus (DMM) and running. These data will be analyzed with multi-body dynamics to estimate the
Last updated on: 08-11-2019 - 14:02
Contact: Judith Piet
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development
A compound of interest (e.g. new psychoactive substance, endocrine disrupting compound, ...) is incubated with human liver microsomes and liver cytosolic fractions to generate both Phase I and II metabolites. Samples are prepared for analysis using a simple method in order to avoid possible losses
Last updated on: 08-11-2019 - 14:01
Contact: Philippe Vervliet
Organisation: University of Antwerp (UAntwerpen)
Status: Published in peer reviewed journal
