The in vitro Alkaline Comet Assay is a microgel electrophoresis technique which allows to measure DNA damage (single and double strand breaks, alkali labile sites, incomplete excision repair sites and cross links) cell by cell. Cells are mixed with 0.8% Low Melting Point Agarose which is spread as a

The Ames test is a short-term bacterial reverse gene mutation assay specifically designed to detect a wide range of chemical substances that can produce genetic damage that leads to permanent gene mutations. The test has been described in detail in OECD TG 471 and employs several histidine dependent

Alkaline comet assay (COM) and Micronucleus test (MN) are well-established cytogenetic tests that are used to detect both immediate damages i.e. DNA fragmentation, and permanent damage which results in micronuclei formation. These techniques are often applied separately or, sometimes, in combination

The cardiorespiratory model reproduces human physiology with a high level of fidelity. It is a lumped parameter model including a representation of atria, ventricles, pulmonary and systemic circulations, autonomic controls, metabolic peripheral control, ventilation and gas exchange in tissues and

We use stem cell-derived sensory neurons and via a small molecule-based protocol (Young et al, 2014) we derive them into human sensory neurons with a nociceptor phenotype (express somatosensory ion channels).