in vitro MDSC-T cell immunosuppression assay

Scope of the method

The Method relates to
  • Human health
The Method is situated in
  • Translational - Applied Research
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Animal derived cells / tissues / organs
Species from which cells/tissues/organs are derived
Type of cells/tissues/organs
Tcells derived from spleen of naive mice and Myloid cells derived from bone marrow of naive mice


Method keywords
  • in vitro assay
  • Coculture
  • immune response
  • flow cytometry
  • cell proliferation
Scientific area keywords
  • Oncology
  • Immunology
  • cancer treatment
  • immunotherapy
Method description

Myeloid progenitor cells derived from the bone marrow of mice are stimulated using a cytokine mixture to become myeloid derived suppressor cell (MDSC) like cells. These are co-cultured with activated CD8+ T-cells derived from a mouse spleen. The MDSC like cells will suppress the proliferation of the T-cells in co-culture which can be observed by staining the CD8+ T-cells using a proliferation dye and flow cytometry. This assay can then be used to screen immunotherapeutic compounds on their capacity to reduce the immunosuppressive effects of MDSC.

Lab equipment
  • Sterile cell culture lab
  • Flow cytometer
Method status
  • History of use
  • Published in peer reviewed journal

References, associated documents and other information


Baert et al. Front Immunol 2019

Associated documents

Contact person

Yani Berckmans


Katholieke Universiteit Leuven (KUL)
Laboratory of Tumor Immunology and Immunotherapy

Oncology - KU Leuven
Laboratory of Tumor Immunology and Immunotherapy