Vectra® Polaris™ Automated Quantitative Pathology Imaging System

Commonly used acronym: Polaris

Scope of the method

Alternative method relates to
  • Human health
Alternative method is situated in
  • Basic Research
  • Translational - Applied Research
Type of alternative method
  • In vitro - Ex vivo
This method makes use of
  • Other (e.g. bacteria)
Human and mice tissues

Description

Method keywords
  • multiplex immunohistochemistry
  • multispectral Imaging
  • FFPE tissue sections
  • TMAs
  • inForm Tissue Finder
Scientific area keywords
  • Immunology
  • Oncology
  • Quantitative pathology
  • Biomarkers
Method description

The Vectra® Polaris™ Automated Quantitative Pathology Imaging System integrates both multispectral imaging and automated slide scanning to better visualize, analyze, quantify, and phenotype immune cells in situ in FFPE tissue sections and TMAs.

Lab equipment

The visualization of multiplex IHC requires a multispectral imaging system.

Method status
  • Internally validated

Pros, cons & Future potential

Advantages

State of the art multispectral imaging enables the identification and downstream quantification of multiple overlapping biomarkers (up to 6) without the interference of autofluorescence as the signals are unmixed from one another. Integrated inForm and phenoptr tissue analysis software packages support configurable projects for biomarker quantification and spatial analysis.

Challenges

Time consuming

Modifications

The acquisition of a fully automated research stainer is planed in a near futur to standardize the staining.

Future & Other applications

The Vectra Polaris Automated Quantitative Pathology Imaging System is a cutting-edge tool for cancer immunology research, but also for apoptosis and/or proliferation assays, necrosis and fibrosis using conventional stains, cell cycle characterization, DNA damage determination, inflammation, autoimmune diseases, transplant acceptance, neurodegenerative diseases, and many more!

References, associated documents and other information

Associated documents
PRD_013272A_01_Vectra_Polaris.pdf

Contact person

Soizic Garaud

Organisations

Institut Jules Bordet
Molecular Immunology Unit
Belgium