Culturing HEK 293 FT cells

Scope of the method

Alternative method relates to
  • Human health
Alternative method is situated in
  • Basic Research
  • Translational - Applied Research
Type of alternative method
  • In vitro - Ex vivo
This method makes use of
  • Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
Human embryonic kidney 293 FT cells

Description

Method keywords
  • Culturing
  • Transfection
  • Viral production
  • High viral titer
Scientific area keywords
  • Viral production
  • High viral titer
  • Clinical translation
  • Cellular reprogramming
Method description

Human embryonic kidney (HEK) 293 FT cells is a celline that is very easy to culture and is used to obtain high viral titers. “293” is a reference to the 293th experiment wherein the cell line was discovered. A transfection with an adenovirus type 5 DNA fragment took place, causing the cell line to express E1A adenoviral gene. This stimulates the transcription of specific viral genes, resulting in a high production of viral proteins. “T” means that the HEK293 cell line is transfected with the SV40 T antigen, also stimulating the production of viral proteins. “F” stands for a fast growing HEK 293T strain with a high transfection efficiency.

Lab equipment
  • Biosafety cabinet
  • Microscope
  • Incubator
Method status
  • History of use

Pros, cons & Future potential

Advantages
  • High viral titer
  • Easy to culture
  • Fast growing
  • Easy to transfect
Challenges

Use of serum

References, associated documents and other information

Associated documents

Contact person

Matthias Rombaut

Organisations

Vrije Universiteit Brussel
Pharmaceutical and Pharmacological Sciences (FARM)
In Vitro Toxicology and Dermato-cosmetology
Belgium