Chronic cardiotoxicity testing in human-induced pluripotent stem cell-derived cardiomyocytes using impedance and multielectrode array

Commonly used acronym: Chronic cardiotoxicity testing in hiPSC-CMs using impedance and MEA

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Scope of the method

The Method relates to
  • Human health
The Method is situated in
  • Translational - Applied Research
  • Other
Safety pharmacology/toxicology testing
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
human induced pluripotent stem cell-derived cardiomyocytes

Description

Method keywords
  • in vitro assay
  • multi electrode array
  • predictivity
  • hiPSC-CMs
Scientific area keywords
  • drug-induced cardiotoxicity
  • arrhythmias
  • Cardiac electrophysiology
  • Drug safety
Method description

Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were seeded in 48-well multielectrode array (MEA) plates and were treated with four doses of reference compounds (covering and exceeding clinical free plasma peak concentrations) and MEA recordings were conducted for 4 days. Functional-electrophysiological (field-potentials) and viability (impedance) parameters were recorded with a MEA machine.

Lab equipment

Multielectrode array

Method status
  • Internally validated
  • Published in peer reviewed journal

Pros, cons & Future potential

Advantages

Human-based model, repeated and non-invasive recordings, chronic

Challenges

Cell maturation

References, associated documents and other information

References

Altrocchi C, Van Ammel K, Steemans M, Kreir M, Tekle F, Teisman A, Gallacher DJ and Lu HR (2023) Evaluation of chronic drug-induced electrophysiological and cytotoxic effects using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Front. Pharmacol. 14:1229960. doi: 10.3389/fphar.2023.1229960

Organisations

Janssen Pharma of JNJ
Research and Development
Belgium
Flemish Region