Cerebellar brain slice model

Scope of the method

Alternative method relates to
  • Animal health
  • Human health
Alternative method is situated in
  • Basic Research
Type of alternative method
  • In vitro - Ex vivo
This method makes use of
  • Animal derived cells / tissues / organs
Species from which cells/tissues/organs are derived
Mus Musculus
Type of cells/tissues/organs
Brain (cerebellum)

Description

Method keywords
  • brainslices
  • cell culture
  • isolation
  • mouse
Scientific area keywords
  • basic research
  • fundamental research
  • neuroscience
  • myelin
Method description

This method describes the steps from a living mouse to a multicellular brain slice model where complex cellular interactions can be evaluated

Method status
  • Still in development

Pros, cons & Future potential

Advantages

By maintaining brain morphology and ultrastructurally the brain cells present, a complex multicellular system is being formed where the interplay between different cells can be evaluated to identify novel remyelinating therapeutics, targets,…

Challenges

Interspecies differences, terminal experiment for the lab animal, requires a training period due to the susceptibility of the brain slices to cell death

References, associated documents and other information

Associated documents
Cerebellar brain slices.docx

Contact person

Melissa Schepers

Organisations

Hasselt University
Biomed Neuro-Immune Connection and Repair
Belgium