Cell-based intestinal absorption models combined with food and digestive matrixes to study toxicity and in vitro bioavailability of food bioactives and contaminants
Commonly used acronym: bioavailability
Scope of the method
The Method relates to
- Human health
The Method is situated in
- Basic Research
Type of method
- In vitro - Ex vivo
This method makes use of
- Human derived cells / tissues / organs
Specify the type of cells/tissues/organsintestine, liver, immune cells
- epithelial barrier function
Scientific area keywords
- effect of food matrix on availability of compounds
A set of protocols to combine the widely used Caco-2 cell line with digests from in vitro digestion models (small intestine, colon) to study toxicity, intestinal barrier integrity, bioavailability and, when combined with other cell models (immune, liver, endothelium), bioactivity of food related bioactives and contaminants.
- - Cell culture facilities;
- - Trans-epithelial electrical resistance measurements;
- - Fluorescence plate reader;
- - Advanced analytical techniques.
- History of use
- Published in peer reviewed journal
Pros, cons & Future potential
- - Includes relevant food and digestive matrices;
- - Barrier and transport assays combined.
- - Case-per-case optimization;
- - Toxicity.
References, associated documents and other information
Van Rymenant, E., Salden, B., Voorspoels, S., Jacobs, G., Noten, B., Pitart, J., Possemiers, S., Smagghe, G., Grootaert, C., Van Camp, J. A critical evaluation of in vitro hesperidin 2S bioavailability in a model combining luminal (microbial) digestion and Caco-2 cell absorption in comparison to a randomized controlled human trial. 2018. MOLECULAR NUTRITION & FOOD RESEARCH. 62(8).
Contact personCharlotte Grootaert
OrganisationsGhent University (UGent)
Food Technology, Safety and Health