Advanced pyrogen/allergen detection through impedance analysis

Scope of the method

The Method relates to
  • Animal health
  • Human health
The Method is situated in
  • Basic Research
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
Cells of the immune system


Method keywords
  • alternative to rabbit pyrogen test
  • advanced impedance spectroscopy
Scientific area keywords
  • allergy
  • pyrogenicity
Method description

Fever-inducing substances need to be traced in medical packaging material. To do so, in the past, rabbits were often exposed to these substances. Causing enormous amounts of animal suffering. Nowadays this test has been largely replaced by the so-called LAL (Limulus amebocyte lysate) test, which uses the blood of the horseshoe crab. This has however led to severe endangerment of this prehistoric species. It is for this reason that mainly colorimetric methods have been developed to detect secondary messenger molecules that are released after immune cells are exposed to pyrogens. However, these in vitro detection methods are expensive and require many hours of preparatory work. In comparison, CellSine's detection method, based on broad-spectrum electrical impedance measurements, is label-free and requires no additional chemicals or pretreatments. Therefore it can be a viable alternative for the LAL test.

Lab equipment

Basic laboratory equipment (biosafety cabinet, mammalian cell incubator, ...)

Method status
  • Still in development

Pros, cons & Future potential

  • - Label-free;
  • - Real-time;
  • - Easy assay development;
  • - Costs.

References, associated documents and other information


Anticancer activity study of chromone and coumarin hybris using electrical impedance spectroscopy (Bouhenna et al., 2018)

Contact person

Eddy-Tim Verjans


Flemish Region