Primary human bronchial and nasal epithelial culture in air liquid interface

Commonly used acronym: ALI-HBEC, ALI-HNEC

Scope of the method

The Method relates to
  • Human health
The Method is situated in
  • Basic Research
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
Bronchi, nasal and sinusal tissues


Method keywords
  • HBEC
  • HNEC
  • ALI
  • air-liquid interface
  • primary culture
  • respiratory epithelium
Scientific area keywords
  • lung
  • bronchus
  • sinus
  • nasal polyps
  • chronic rhinosinusitis
  • cystic fibrosis
  • allergic rhinitis
  • Chronic obstructive pulmonary disease
  • human airways
Method description

After digestion with pronase, human epithelial cells are cultured on flask and then on inserts to recapitulate a fully differentiated epithelium.

Method status
  • Published in peer reviewed journal

Contact person

Sophie Gohy


Cliniques universitaires Saint-Luc, UCLouvain