Primary human bronchial and nasal epithelial culture in air liquid interface
Commonly used acronym: ALI-HBEC, ALI-HNEC
Scope of the method
The Method relates to
- Human health
The Method is situated in
- Basic Research
Type of method
- In vitro - Ex vivo
This method makes use of
- Human derived cells / tissues / organs
Specify the type of cells/tissues/organs
Bronchi, nasal and sinusal tissues
Description
Method keywords
- HBEC
- HNEC
- ALI
- air-liquid interface
- primary culture
- respiratory epithelium
Scientific area keywords
- lung
- bronchus
- sinus
- nasal polyps
- chronic rhinosinusitis
- cystic fibrosis
- allergic rhinitis
- Chronic obstructive pulmonary disease
- human airways
Method description
After digestion with pronase, human epithelial cells are cultured on flask and then on inserts to recapitulate a fully differentiated epithelium.
Method status
- Published in peer reviewed journal
References, associated documents and other information
Associated documents
Colin et al. 2020. Ebiom. Lung IgA in CF..pdf
Gohy et al. 2015. ERJ. EMT and COPD.pdf
Gohy. 2014. Ajrccm. pIgR and COPD.pdf
Carlier 2020. Ebiom.pdf
Contact person
Sophie GohyOrganisations
Cliniques universitaires Saint-Luc, UCLouvainIREC, PNEU
Belgium