Primary cortical neuron isolation

Scope of the method

The Method relates to
  • Animal health
  • Human health
The Method is situated in
  • Basic Research
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Animal derived cells / tissues / organs
Species from which cells/tissues/organs are derived
Mus Musculus
Type of cells/tissues/organs
Brain (cortex)

Description

Method keywords
  • cell culture
  • isolation
  • Cortex
  • Neurons
Scientific area keywords
  • basic research
  • neuroscience
  • fundamental research
  • neurodevelopment
Method description

This method describes the steps from a living mouse to a single cell solution of primary cortical neurons.

Method status
  • Internally validated

Pros, cons & Future potential

Advantages
  • Primary cultures give rise to a condition more similar although not identical to the in vivo situation when compared to cell lines ;
  • Methodologically feasible ;
  • Wide application range within the field of neuroscience.
Challenges
  • Interspecies differences ;
  • Terminal experiment for the lab animal.
Future & Other applications

Adjustment of the protocol can be made to isolate primary neurons of other species.

References, associated documents and other information

Associated documents
Primary cortical neurons.docx

Contact person

Melissa Schepers

Organisations

Hasselt University
Biomed Neuro-Immune Connection and Repair
Belgium
Flemish Region