Ibidi flow system immune cell adhesion assay

Commonly used acronym: Adhesion assay

Scope of the method

The Method relates to
  • Animal health
  • Human health
The Method is situated in
  • Basic Research
  • Translational - Applied Research
Type of method
  • In vitro - Ex vivo
This method makes use of
  • Animal derived cells / tissues / organs


Method keywords
  • adhesion
  • endothelial cells
  • migration
  • shear stress
  • blood brain barrier
  • blood flow
Scientific area keywords
  • immune cell migration
  • human white blood cells
  • blood brain barrier
  • multiple sclerosis
  • cell adhesion
  • T lymphocyte
  • blood vessel
Method description

Using an in vitro flow system adhesion assay, immune cell adhesion to (blood-brain barrier) endothelial cells can be quantified under physiological blood flow conditions. Endothelial cells can be treated with inflammatory cytokines or therapeutic antibodies to mimic inflammatory diseases. Immune cell adhesion (including rolling, probing or crawling) can be visualized using live-cell imaging.

Lab equipment

Ibidi pump system and slides to mimic physiological blood flow

Method status
  • Published in peer reviewed journal

Pros, cons & Future potential

  • - Detailed analysis of migration phenotype (rolling, crawling, probing)
  • - Compare different genotypes or treatment conditions
  • - Optimization required
  • - Time consuming data acquisition and analysis

References, associated documents and other information

Oncostatin M triggers brain inflammation by compromising blood-brain barrier in…

Contact person

Doryssa Hermans


University of Hasselt (UHasselt)
Department of Immunology and Infection
Flemish Region