Human in vitro liver metabolism using HLM, HLCYT and Liquid Chromatography coupled to High-Resolution Mass Spectrometry

A compound of interest (e.g. new psychoactive substance, endocrine disrupting compound, ...) is incubated with human liver microsomes and liver cytosolic fractions to generate both Phase I and II metabolites. Samples are prepared for analysis using a simple method in order to avoid possible losses of biotransformation products. The extracts are analysed using liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. Identification of the biotransformation products is performed using complementary screening workflows. These include a suspect screening based on in silico predictions and non-targeted screening using either vendor-specific or in-house developed open-source software protocols.