Differentiation of ovarian stromal cells to steroidogenic theca interna cells
Scope of the method
- Human health
- Basic Research
- In vitro - Ex vivo
- Animal derived cells / tissues / organs
- theca cells
- Ovarian cells
- fertility restoration
- artificial ovary
- Human ovarian tissue
We aim to differentiate ovarian cells isolated from postmenopausal ovaries into steroidogenic theca interna cells. For this purpose, cryopreserved ovarian cortex fragments are used to isolate ovarian cells. These cells are cultured on collagen and supplemented with growth factors defined to promote differentiation into theca interna cells. After 8 days of culture, theca interna cells are obtained, characterized by steroidogenic enzymes such as CYP17A1, and by the production of steroidogenic hormones.
- - Tissue chopper;
- - Biosafety cabinet;
- - Incubator;
- - Centrifuge;
- - Water bath with shaker
- Published in peer reviewed journal
Pros, cons & Future potential
In vitro differentiation and culture of theca interna cells.
Theca cells are obtained after 8 days of culture, however, they lose their characteristics when kept in culture for a longer time period.
Yes, the pool of cell types within ovarian cells can be reduced to only those that undergo differentiation.
References, associated documents and other information
P Asiabi, M M Dolmans, J Ambroise, A Camboni, C A Amorim, In vitro differentiation of theca cells from ovarian cells isolated from postmenopausal women, Human Reproduction, Volume 35, Issue 12, December 2020, Pages 2793–2807, https://doi.org/10.1093/humrep/deaa246
Contact personHanne Vlieghe
OrganisationsUniversité Catholique de Louvain (UCL)