Culture of human duodenal epithelial stem cells as organoids

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Scope of the method

The Method relates to

Human health

The Method is situated in

Basic Research
Translational - Applied Research

Type of method

In vitro - Ex vivo

This method makes use of

Human derived cells / tissues / organs

Specify the type of cells/tissues/organs

duodenal epithelial stem cells/biopsies

Description

Method keywords

transepithelial electric resistance
cell permeability
gut organoids
epithelium

Scientific area keywords

gastroenterology
Intestinal organoids
lipid metabolism

Method description

Human duodenal biopsies from metabolic dysfunction-associated steatohepatitis (MASH) patients were used to generate organoids and then investigate potential alterations in intestinal barrier and absorptive functions.

Method status

Published in peer reviewed journal

References, associated documents and other information

References

Duodenal organoids from metabolic dysfunction-associated steatohepatitis patients exhibit absorptive and barrier alterations Hadefi, Alia et al. Gastro Hep Advances, Volume 0, Issue 0, 100599

Associated documents

Hadefi et al Gastro hep advances 2024.pdf