Cornea-on-chip

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Scope of the method

The Method relates to

Human health

The Method is situated in

Basic Research
Regulatory use - Routine production
Translational - Applied Research

Type of method

In vitro - Ex vivo

This method makes use of

Human derived cells / tissues / organs

Specify the type of cells/tissues/organs

Corneal cells

Description

Method keywords

cornea-on-chip
Cornea
organ-on-chip
microfluidics

Scientific area keywords

Tissue engineering
in vitro 3D modelling
Ophtalmology
pharmacokinetics
Extracellular matrices

Method description

The main objective of this research project is to create the first full-thickness cornea-on-chip, which comprises a 3D construct with every cellular layer of the cornea. This construct is embedded in a microfluidic chip with two channels that are continuously perfused. The epithelial side of the construct is exposed to an artificial tear film and the endothelial side is connected to an artificial anterior chamber. The former allows injection of ophthalmic formulations and simulates the physiology of the tear film while the latter can be used for sampling and providing nutrients.

Lab equipment

Pumps

Method status