ATP cell viability assay
Scope of the method
- Human health
- Basic Research
- In vitro - Ex vivo
- Human derived cells / tissues / organs
- cell viability test
- in vitro
- in vitro cytotoxicity
The ATP cell viability assay (CellTiter-Glo assay test kit) provides a quantitative measurement of the ATP content directly proportional to the number of cells present in culture. The homogeneous assay procedure involves adding one single reagent, CellTiter-Glo Reagent directly to cells cultured in serum-supplemented medium. This results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. Luminescence can be measured already 10 minutes after adding reagent & mixing. The half-life of the luminescent signal is greater than five hours so the measurement can be performed even hours after adding the reagent. The CellTiter-Glo® Assay relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase), which generates a stable “glow-type” luminescent signal. % Cell viability after exposure is calculated through comparison of measured RLU of unexposed control cells (set to 100% viability) and exposed cells.
Standard equipment for working with cell cultures, luminometer
- History of use
- Published in peer reviewed journal
Pros, cons & Future potential
- Sensitive (even few cells can be detected),
- Fast (data available 10 minutes after adding reagent),
- Very stable luminescent signal flexible (ATP content from both adherent and suspension cells in different well formats can be measured),
- No interference with compounds with oxido-reductive potential (MTT) or colored compounds (NRU).
- Expensive (test kit),
- Sensitivity can be a disadvantage (the occurrence of metabolic oscillations can cause fluctuations of the ATP content per cells).
References, associated documents and other information
J Shi, S Springer, P Escobar (2010) Coupling cytotoxicity biomarkers with DNA damage assessment in TK6 human lymphoblast cells 10.1016/j.mrgentox.2010.01.008
Chemical and physical health risks