Vascular smooth muscle cell (VSMC) deficiency plays a pivotal role in aneurysm development. Unfortunately, access to native VSMCs of patients and (particularly) control individuals is extremely limited. It has been shown that iPSC-derived VSMCs recapitulate the yet known disease processes very well.

Last updated on: 03-06-2020 - 13:51

Organisation: University of Antwerp (UAntwerpen)
Status: Still in development, Internally validated
This method is a straightforward, colorimetric, and inexpensive high-throughput screening system in bacteria which depends on the activity of human HPD. This screening assay is based on the formation and accumulation of a melanin-like ochronotic pigment which has a characteristic brown color. In the

Last updated on: 26-05-2020 - 11:30

Organisation: , RWTH Aachen
Partners: RWTH Aachen
Status: Published in peer reviewed journal
Amoebae are natural eukaryotic predators of bacteria, yeasts, fungi and they are ubiquiste. They are excellent and easy-to-use cellular infection models, as they allow to co-cultivate any organisms in a broad range of infection medium, compatible with high quality microscopy techniques,

Last updated on: 19-05-2020 - 11:02

Organisation:
Status: Internally validated, Published in peer reviewed journal
A calculated number of bacteria is injected through the pro-legs of the larvae. As several entry points are present, this allows to inject virulent bacteria and potential antimicrobial compounds within the same host. The survival of the larvae is scored over time (days post inoculation) until about

Last updated on: 19-05-2020 - 11:01

Organisation:
Status: Internally validated, Published in peer reviewed journal
Reverse pharmacology is a high-throughput in vitro method to characterise ligand-receptor interactions. In this method, a receptor of interest is expressed in a heterologous cell line and used as a hook to fish out its ligand(s) from a library of synthetic compounds. Receptor activation is measured

Last updated on: 17-04-2020 - 09:56

Contact: Isabel Beets
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The nematode Caenorhabditis elegans provides a powerful model system to study fundamental working mechanisms of the nervous system in a living animal. It's main advantages are its compact nervous system that has been fully mapped, its short generation time and amenability for genetic research. As a

Last updated on: 17-04-2020 - 09:55

Contact: Isabel Beets
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Computational Fluid Dynamics (CFD) is being applied to characterize the fluid flow in different applications. CFD has obtained significant interest in both the medical and engineering community because of its non-invasive character. It can predict the fluid flow characteristics when one or multiple

Last updated on: 10-04-2020 - 09:22

Organisation: Ghent University (UGent)
Status: Still in development, Published in peer reviewed journal
Performing biopredictive dissolution tests in in vitro models that are frequently used in pharmaceutical and academic institutions and using these in vitro dissolution data as input for PBPK models to predict the systemic exposure of the drug in humans/patients.

Last updated on: 08-04-2020 - 17:29

Contact: Bart Hens
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We study the influence of food parameters on the digestive kinetics of diverse nutrients throughout the upper gastrointestinal tract. For this, we use a static in vitro digestion protocol based on the standardized, consensus method recommended by the INFOGEST consortium. Briefly, the food is first

Last updated on: 31-03-2020 - 21:01

Contact: Tara Grauwet
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: INFOGEST consortium
Status: Published in peer reviewed journal
The neutral red uptake (NRU) assay provides a quantitative measurement of the number of viable cells. The test is based on the ability of living cells to take up and bind neutral red (NR), a dye which easily penetrates cell membranes via non-ionic diffusion and accumulates in the lysosomes.

Last updated on: 27-03-2020 - 14:53

Contact: Roel Anthonissen
Organisation:
Status: History of use, Published in peer reviewed journal