Alkaline comet assay (COM) and Micronucleus test (MN) are well-established cytogenetic tests that are used to detect both immediate damages i.e. DNA fragmentation, and permanent damage which results in micronuclei formation. These techniques are often applied separately or, sometimes, in combination
Last updated on: 18-02-2022 - 16:42
Contact: Ha Nguyen
Organisation: Université Libre de Bruxelles (ULB), Sciensano
Partners: University of Liège (ULiège)
Status: History of use, Internally validated
A set of protocols to combine the widely used Caco-2 cell line with digests from in vitro digestion models (small intestine, colon) to study toxicity, intestinal barrier integrity, bioavailability and, when combined with other cell models (immune, liver, endothelium), bioactivity of food related
Last updated on: 18-02-2022 - 08:42
Contact: Charlotte Grootaert
Organisation: Ghent University (UGent)
Status: History of use, Published in peer reviewed journal
Using respirometry (oxygen and pH, XF96 Analyzer), computer assisted cell analysis, and specific substrates and stressors, mitochondrial function and metabolic changes in a diverse set of cell lines can be measured. Relevant to study substrate preferences, acute and chronic effects of toxic
Last updated on: 15-02-2022 - 15:31
Contact: Charlotte Grootaert
Organisation: Ghent University (UGent)
Status: History of use, Published in peer reviewed journal
Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase
Last updated on: 11-02-2022 - 10:29
Contact: Olivier De Wever
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Advances in cell culture methods allowed 3D culture of gut epithelial cells derived from adult intestinal epithelial stem cells in mice and humans. We show that starting from crypts cultured in an extracellular matrix hydrogel together with essential growth factors, gut organoids can be grown from
Last updated on: 20-01-2022 - 16:14
Contact: Bert Devriendt
Organisation: Ghent University (UGent)
Status: Still in development, Published in peer reviewed journal
Advances in three-dimensional (3D) culture techniques have shown several advantages over 2D cultures, especially by more accurately mimicking the in vivo environment. This has led to improved reproducibility and reliability of experimental results, which are important criteria in disease modelling
Last updated on: 14-10-2021 - 16:59
Contact: Jolanda van Hengel
Organisation: Ghent University (UGent)
Status: Still in development, Published in peer reviewed journal
The response of sublingual epithelial cells to house dust mite allergen and potential tolerance-promoting adjuvants such as Toll-like receptor (TLR) ligands and calcitriol was investigated using primary sublingual epithelial cells isolated from dogs and cultured in vitro. After 24-h incubation with
Last updated on: 07-04-2021 - 14:52
This is a mathematical compartmental formulation of dose-effect synergy modelling for multiple therapies in Non Small Cell Lung Cancer (NSCLC): antiangiogenic, immuno- and radiotherapy. The model formulates the dose-effect relationship in a unified context, with tumor proliferating rates and
Last updated on: 01-02-2021 - 14:32
Contact: Clara Mihaela Ionescu
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Computational Fluid Dynamics (CFD) is being applied to characterize the fluid flow in different applications. CFD has obtained significant interest in both the medical and engineering community because of its non-invasive character. It can predict the fluid flow characteristics when one or multiple
Last updated on: 10-04-2020 - 09:22
Contact: Mohammad Rahimi Gorji
Organisation: Ghent University (UGent)
Status: Still in development, Published in peer reviewed journal
Retinal gene delivery via intravitreal injection is hampered by various physiological barriers present in the eye of which the vitreoretinal (VR) interface represents the most serious hurdle. We present a retinal explant model especially designed to study the role of this interface as a barrier for
Last updated on: 24-03-2020 - 16:26
Contact: Katrien Remaut
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
